Endothelin-1-induced increases in microvascular permeability in isolated, perfused rat lungs requires leukocytes and plasma.
Endothelin-1-induced increases in microvascular permeability in isolated, perfused rat lungs requires leukocytes and plasma.
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在离体、灌注的大鼠肺中，内皮素-1 诱导的微血管通透性增加需要白细胞和血浆。
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
A. Hauge
中科院分区：
文献类型：
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作者：
E. Helset;J. Kjaeve;A. Hauge
研究了内皮素-1 (ET-1) （10(-8) M）对大鼠离体肺灌注血液或各种血液成分时肺微血管通透性的影响。为了防止血管平滑肌张力的改变，通过测量经罂粟碱预处理的肺的液体滤过率（FFR）来评估微血管通透性。ET-1显著提高血液灌注60 min后FFR （131.0 +/- 10.1 mg/min, P < 0.01）。在血浆中重悬白细胞灌注的肺中，ET-1在灌注液中加入30 min （40.4 +/- 11.4 mg/min, P < 0.01）和60 min （97.4 +/- 14.5 mg/min, P < 0.01）后均显著提高FFR。血浆热失活（56℃，1小时）没有减弱ET-1的这种作用（94.4 +/- 25.1 mg/min, P < 0.01）。当肺部灌注克雷伯林格白蛋白重悬的白细胞而不是血浆，或仅用血浆灌注时，ET-1未引起FFR的任何变化。综上所述，ET-1增加了离体血灌注大鼠肺微血管通透性。这种作用主要依赖于补体以外的白细胞和血浆成分的存在。
Effect of endothelin-1 (ET-1) (10(-8) M) on pulmonary microvascular permeability was examined in isolated rat lungs perfused with blood or various blood components. Microvascular permeability was assessed by measuring fluid filtration rate (FFR) in lungs pretreated with papaverine in order to prevent changes in vascular smooth muscle tone. ET-1 significantly increased FFR (131.0 +/- 10.1 mg/min, P < 0.01) after perfusion with blood for 60 min. In lungs perfused with leukocytes resuspended in plasma, ET-1 increased FFR significantly both 30 min (40.4 +/- 11.4 mg/min, P < 0.01) and 60 min (97.4 +/- 14.5 mg/min, P < 0.01) after it was added to the perfusate. Heat inactivation (56 degrees C; 1 hr) of plasma did not attenuate this effect of ET-1 (94.4 +/- 25.1 mg/min, P < 0.01). When lungs were perfused with leukocytes resuspended in Krebs Ringer albumin instead of plasma, or with plasma only, ET-1 did not cause any change in FFR. In conclusion, ET-1 increases microvascular permeability in isolated blood-perfused rat lungs. The effect is critically dependent on the presence of leukocytes and plasma components other than complement.