Measurements of the self-assembly kinetics of individual viral capsids around their RNA genome
Measurements of the self-assembly kinetics of individual viral capsids around their RNA genome
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DOI：
10.1073/pnas.1909223116
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发表时间：
2019-11-05
影响因子：
11.1
通讯作者：
Manoharan, Vinothan N.
中科院分区：
文献类型：
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作者：
Garmann, Rees F.;Goldfain, Aaron M.;Manoharan, Vinothan N.
自组装被生物系统广泛用于构建功能性纳米结构，例如RNA病毒的蛋白质衣壳。但是，由于组装是一种集体现象，涉及许多弱相互作用的亚基和广泛的时间尺度，组装途径的测量一直是难以捉摸的。我们使用干涉散射显微镜来测量单个MS2噬菌体衣壳围绕MS2 RNA的组装动力学。通过记录有多少外壳蛋白结合到许多单独的RNA链中的每一条上，我们发现组装是通过成核然后单调生长进行的。我们的测量揭示了组装途径的定量细节，并显示其故障模式。我们使用这些结果来严格审查模型的组装过程。
Self-assembly is widely used by biological systems to build functional nanostructures, such as the protein capsids of RNA viruses. But because assembly is a collective phenomenon involving many weakly interacting subunits and a broad range of timescales, measurements of the assembly pathways have been elusive. We use interferometric scattering microscopy to measure the assembly kinetics of individual MS2 bacteriophage capsids around MS2 RNA. By recording how many coat proteins bind to each of many individual RNA strands, we find that assembly proceeds by nucleation followed by monotonic growth. Our measurements reveal the assembly pathways in quantitative detail and also show their failure modes. We use these results to critically examine models of the assembly process.