Nucleotide Sequence Requirements at the 5′ End of the Influenza A Virus M RNA Segment for Efficient Virus Replication
Nucleotide Sequence Requirements at the 5′ End of the Influenza A Virus M RNA Segment for Efficient Virus Replication
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DOI：
10.1128/jvi.02513-08
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发表时间：
2009-04-01
影响因子：
5.4
通讯作者：
Kawaoka, Yoshihiro
中科院分区：
文献类型：
--
作者：
Ozawa, Makoto;Maeda, Junko;Kawaoka, Yoshihiro
甲型流感病毒基因组包装成病毒体的机制尚未完全了解。除了M片段外，病毒RNA片段包装所需的编码区和非编码区已经确定。在这里，我们描绘的M段区域，通过将报告病毒RNA纳入病毒粒子，并通过产生具有突变的区域中的病毒。我们发现，与其他片段一样，M片段编码区对于病毒体掺入是必不可少的，并且编码区5'端的核苷酸长度而不是核苷酸序列是重要的。
The mechanism by which the influenza A virus genome is packaged into virions is not fully understood. The coding and noncoding regions necessary for packaging of the viral RNA segments, except for the M segment, have been identified. Here, we delineate the M segment regions by incorporating a reporter viral RNA into virions and by generating viruses possessing mutations in the regions. We found that, like the other segments, the M segment coding regions are essential for virion incorporation and that the nucleotide length rather than the nucleotide sequence of the 5' end of the coding region is important.