Puerarin alleviates aggravated sympathoexcitatory response induced by myocardial ischemia via regulating P2X3 receptor in rat superior cervical ganglia
Puerarin alleviates aggravated sympathoexcitatory response induced by myocardial ischemia via regulating P2X3 receptor in rat superior cervical ganglia
复制标题
葛根素通过调节大鼠颈上神经节P2X3受体减轻心肌缺血引起的交感兴奋反应加重
DOI：
10.1016/j.neuint.2014.03.004
复制
发表时间：
2014-05-01
影响因子：
4.2
通讯作者：
Liang, Shangdong
中科院分区：
文献类型：
--
作者：
Liu, Shuangmei;Yu, Shicheng;Liang, Shangdong
心肌缺血引起以血压升高和交感神经活动为特征的交感兴奋反应。葛根素是从中草药葛根中提取的主要活性成分，广泛用于治疗心肌和脑缺血。然而，人们对这种机制知之甚少。本研究旨在探讨葛根素对心肌缺血损伤所致交感兴奋反应的影响及其与P2X(3)受体的可能关系。结果表明，葛根素能降低心肌缺血大鼠的收缩压和心率，并能抑制心肌缺血大鼠SCG内P2X(3)基因和蛋白的上调。心肌缺血组SCG神经元ATP激活电流幅度明显大于对照组。葛根素降低心肌缺血组和对照组的ATP激活电流，且心肌缺血组的抑制作用强于对照组。葛根素还能显著抑制转染P2X(3)受体的HEK293细胞的ATP激活电流。以上结果提示，葛根素可通过作用于大鼠下丘脑脊束核P2X(3)受体而抑制心肌缺血引起的上交感兴奋反应，从而起到保护心肌的作用。(C)2014爱思唯尔有限公司。保留所有权利。
Myocardial ischemia elicits a sympathoexcitatory response characterized by increase in blood pressure and sympathetic nerve activity. Puerarin, a major active ingredient extracted from the traditional Chinese plant medicine Ge-gen, has been widely used in treatment of myocardial and cerebral ischemia. However, little is known about the mechanism. Our study was aimed to explore the effect of puerarin on sympathoexcitatory response induced by myocardial ischemic injury and possible relationship with P2X(3) receptor. Our results showed that puerarin alleviated systolic blood pressure and heart rate, and decreased the up-regulated of P2X(3) mRNA and protein in SCG of myocardial ischemic rats. The amplitude of ATP-activated currents of SCG neurons was much larger in myocardial ischemic group than that in control group. Puerarin reduced ATP-activated currents in myocardial ischemic group and control group, and the inhibiting effects of puerarin in myocardial ischemic group were stronger than those in control group. Puerarin also significantly inhibited ATP-activated currents in HEK293 cells transfected with P2X(3) receptor. These results suggest that puerarin can depress up-sympathoexcitatory response induced by myocardial ischemia via acting on P2X(3) receptor in rat SCG to protect myocardium. (c) 2014 Elsevier Ltd. All rights reserved.