Simultaneous determination of Fe, P, Ca, Mg, Zn, and Cu in whole blood by two-jet plasma atomic emission spectrometry
Simultaneous determination of Fe, P, Ca, Mg, Zn, and Cu in whole blood by two-jet plasma atomic emission spectrometry
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DOI：
10.1016/j.talanta.2009.10.046
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发表时间：
2010-03-15
期刊：
影响因子：
6.1
通讯作者：
Nevinsky, Georgy A.
中科院分区：
文献类型：
--
作者：
Zaksas, Natalia P.;Gerasimov, Vladimir A.;Nevinsky, Georgy A.
用于生物医学研究的大量生物样本的可用性有时非常有限。因此，需要能够分析少量样品的简单技术。在目前的工作中，提出了用于测定全血中 Fe、P、Ca、Mg、Zn 和 Cu 的双射流等离子体原子发射光谱技术。第一种技术是为直接分析冻干血液而开发的。样品制备包括用光谱缓冲液（含有 15 wt% NaCl 的石墨粉）稀释血粉（粒径 20 μm 或更小）。对于液体全血的分析，采用了在石墨粉末上蒸发的血液的预先碳化（不是灰化） 使用基于含有 15 wt.% NaCl 的石墨粉末的校准样品 通过使用不同的样品制备程序（湿式酸消解和干式碳化）、分析 IAEA A-13 标准物质（冻干牛全血）以及结果比较来确认技术的有效性 通过所提出的技术与溶出伏安法技术的结果获得的仅20-50μL全血就足以进行所有测定。该技术成功应用于Fe、P、Ca的同时测定。镁。活体实验大鼠和小鼠全血以及人血中的锌和铜。 (C) 2009 Elsevier B.V. 保留所有权利
Availability of many biological samples in ample quantity for biomedical investigations sometimes is very restricted. Therefore, there is the need for the simple techniques allowing the analysis of small amount samples. In the present work the two-jet plasma atomic emission spectrometry techniques for the determination of Fe, P, Ca, Mg, Zn, and Cu in whole blood are proposed. The first technique is developed for direct analysis of freeze-dried blood. The sample preparation consisted in a dilution of blood powder (particle size 20 mu m or less) with a spectroscopic buffer (graphite powder containing 15 wt % NaCl). For the analysis of liquid whole blood, previous carbonization (not ashing) of blood evaporated on graphite powder was applied Calibration samples based on graphite powder containing 15 wt.% NaCl were used The validation of the techniques was confirmed by the use of different sample preparation procedures (wet acid digestion and dry carbonization), the analysis of IAEA A-13 reference material (freeze-dried bovine whole blood), and the comparison of the results obtained by the proposed technique with the results of the stripping voltammetry technique Just 20-50 mu L of whole blood is quite enough for all determinations. The proposed techniques were successfully applied for the simultaneous determination of Fe, P. Ca. Mg. Zn, and Cu in whole blood of living experimental rats and mice and human blood. (C) 2009 Elsevier B.V. All rights reserved