Superoxide sensor based on hemin modified electrode
Superoxide sensor based on hemin modified electrode
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DOI：
10.1016/s0925-4005(00)00575-x
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发表时间：
2000-11-01
影响因子：
8.4
通讯作者：
Scheller, FW
中科院分区：
文献类型：
--
作者：
Chen, J;Wollenberger, U;Scheller, FW
制备了氯化血红素修饰热解石墨电极，并将其用于超氧阴离子的电化学测定。用循环伏安法测定了吸附态氯化血红素的非均相电子转移速率常数为15 s（-1）。将氯化血红素修饰电极用于检测黄嘌呤氧化酶（XOD）催化次黄嘌呤氧化产生的超氧阴离子自由基。灵敏度高于已建立的基于细胞色素c（cyt c）的传感器。超氧化物歧化酶（SOD）和尿酸的抗氧化活性也进行了研究。(C)2000 Elsevier Science B. V.保留所有权利。
A hemin modified pyrolytic graphite (PG) electrode was prepared and applied for the electrochemical determination of superoxide. The rate constant of heterogeneous electron transfer of adsorbed hemin was determined with cyclic voltammetry (CV) to be 15 s(-1). The hemin modified electrode was applied to detect superoxide radicals produced by xanthine oxidase (XOD) catalyzed hypoxanthine oxidation. Sensitivity was higher than compared to the established cytochrome c (cyt c)-based sensor. The antioxidative activity of superoxide dismutase (SOD) and uric acid was also investigated. (C) 2000 Elsevier Science B.V. All rights reserved.