Prospects for the use of spherical gold nanoparticles in immunization.
Prospects for the use of spherical gold nanoparticles in immunization.
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DOI：
10.1007/s00253-018-9476-5
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发表时间：
2019-01
影响因子：
5
通讯作者：
Guliy OI
中科院分区：
文献类型：
--
作者：
Staroverov SA;Volkov AA;Mezhenny PV;Domnitsky IY;Fomin AS;Kozlov SV;Dykman LA;Guliy OI
近年来，使用通过生物和化学合成制备的纳米结构的新型病毒纳米疫苗和诊断剂的发展非常迅速。我们使用球形金纳米颗粒（平均直径，15 nm）作为平台的抗原猪传染性胃肠炎病毒（TGEV）。文献数据表明，用与金纳米颗粒（GNP）偶联的TGEV抗原免疫动物不仅激活抗原呈递细胞，而且增加脾淋巴（抗体形成）细胞的增殖活性。GNP-抗原结合物免疫动物的γ-IFN、IL-1β和IL-6含量高于完整动物或单独给予抗原的动物。免疫动物中IL-1β浓度的增加与巨噬细胞和刺激的B细胞的活性直接相关，这些细胞在活化时产生这种细胞因子。IL-6浓度的增加表明注射的制剂对细胞免疫有刺激作用。用缀合至GNP作为载体的TGEV抗原免疫激活淋巴细胞和腹膜巨噬细胞的呼吸活性，这与它们的转化活性和抗体产生的激活直接相关。此外，该缀合物的使用允许显著改善动物免疫器官的结构并恢复这些器官的形态-功能状态。显微解剖学变化（滤泡数量增加）表明脾脏B依赖区的激活，因此，体液型免疫反应的发展。在用TGEV抗原单独免疫的动物中观察到的降解过程是对病原体攻击的弱抗性的证据。这些结果可用于开发针对这种感染的疫苗，通过采用TGEV抗原偶联到金纳米颗粒作为载体。
Recent years have seen extremely fast development of new viral nanovaccines and diagnostic agents using nanostructures prepared by biological and chemical synthesis. We used spherical gold nanoparticles (average diameter, 15 nm) as a platform for the antigen for swine transmissible gastroenteritis virus (TGEV). The literature data demonstrate that immunization of animals with the TGEV antigen coupled to gold nanoparticles (GNPs) not only activates antigen-presenting cells but also increases the proliferative activity of splenic lymphoid (antibody-forming) cells. The contents of γ-IFN, IL-1β, and IL-6 in animals immunized with GNP-antigen conjugates were found to be higher than those in intact animals or in animals given the antigen alone. The increased concentration of IL-1β in the immunized animals directly correlated with the activity of macrophages and stimulated B cells, which produce this cytokine when activated. The increased concentration of IL-6 indicates that the injected preparations are stimulatory to cellular immunity. Immunization with the TGEV antigen conjugated to GNPs as a carrier activates the respiratory activity of lymphoid cells and peritoneal macrophages, which is directly related to their transforming activity and to the activation of antibody generation. Furthermore, the use of this conjugate allows marked improvement of the structure of the animals’ immune organs and restores the morphological–functional state of these organs. The microanatomical changes (increased number of follicles) indicate the activation of the B-dependent zone of the spleen and, consequently, the development of a humoral-type immunological reaction. The degradative processes observed in the animals immunized with TGEV antigen alone are evidence of weak resistance to pathogen attack. These results can be used to develop vaccines against this infection by employing TGEV antigen coupled to gold nanoparticles as a carrier.
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DOI：
10.2217/nnm.13.58
发表时间：
2014-02
期刊：
Nanomedicine (London, England)
影响因子：
--
作者：
Tao W;Ziemer KS;Gill HS
通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
3.5
作者：
Chen, Yu-Shiun;Hung, Yao-Ching;Huang, Guewha Steven
通讯作者：
Huang, Guewha Steven
影响因子：
5.8
作者：
Barhate, Ganesh;Gautam, Manish;Pokharkar, Varsha
通讯作者：
Pokharkar, Varsha
影响因子：
46.2
作者：
Mout R;Moyano DF;Rana S;Rotello VM
通讯作者：
Rotello VM