CYTOGENETIC ANALYSIS OF THE 2B3-4-2B11 REGION OF THE X-CHROMOSOME OF DROSOPHILA-MELANOGASTER .1. CYTOLOGY OF THE REGION AND MUTANT COMPLEMENTATION GROUPS
CYTOGENETIC ANALYSIS OF THE 2B3-4-2B11 REGION OF THE X-CHROMOSOME OF DROSOPHILA-MELANOGASTER .1. CYTOLOGY OF THE REGION AND MUTANT COMPLEMENTATION GROUPS
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DOI：
10.1007/bf00285954
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发表时间：
1980-01-01
期刊：
影响因子：
1.6
通讯作者：
ZHIMULEV, IF
中科院分区：
文献类型：
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作者：
BELYAEVA, ES;AIZENZON, MG;ZHIMULEV, IF
在黑腹水蚤X染色体的2B1-2-2C1-2区域，至少有11条明显的单带和1条真正的双带。这一数字与布里奇斯修订后的地图中的数字一致，大多数双线带都是人工制品。该地区出现了三次喷发。早期蜕皮激素专一性喷雾2B5-6，在PS(膨胀期)1时较小，在PS 2时急剧增加，晚期蜕皮激素专性喷雾2C1-2出现在PS 4-5。在PS 9-10，当2B5-6完全消失而2C1-2减少时，在2B11出现第三次喷发。形态分析和~3H-尿苷掺入染色体重排的放射自显影研究表明，早期蜕皮激素特异的膨化产物可能来自2B5和2B6带，而相邻的2B1-4和2B7-10带在所研究的阶段没有明显的转录。在2B5-6喷雾所在的区域，有42个突变影响生存能力。这些突变属于6个互补群，其中Dor和Swi两个群是独立的，其余的被几个致命的突变(重叠复合体)重叠。不同群体的突变体具有一系列相似的特征：温度敏感性、剂量敏感性、对幼虫的致死性和相似的形态异常。在2B区域内可能存在一个功能相连的基因簇。互补基团br、rbp、L(1)pp-1、L(1)pp-2(重叠复合体)通过重排定位在发育泡沫区极窄的细胞学界限2B3-4-2B5内。另外两个基因座Dor和Swi位于2B5的右侧。
There are, at least, 11 distinct single bands and 1 real doublet in the region 2B1-2-2C1-2 of the X-chromosome of D. melanogaster. This figure coincides with that in Bridges'' revised map with most of the doublet bands being artifacts. Three puffs appear in the region. The early ecdysone-specific puff 2B5-6, small at PS(puffing stage) 1, increases sharply at PS 2. The late ecdysone-specific puff 2C1-2 appears at PS 4-5. At PS 9-10, when 2B5-6 disappears completely and 2C1-2 decreases, a third puff at 2B11 appears. None of these puffs in active at PS 11. Morphological analysis of puff appearance and autoradiographic study of 3H-uridine incorporation into chromosomes carrying rearrangements within the 2B region suggest that the early ecdysone-specific puff derives from bands 2B5 and maybe 2B6, while the neighboring bands 2B1-4, and 2B7-10 do not show appreciable transcription at the investigated stages. There are 42 mutations affecting viability in the region where the 2B5-6 puff is located. The mutations belong to 6 complementation groups; 2 of the groups dor and swi, are independent while the rest are overlapped by several lethal mutations (overlapping complex). Mutants of the different groups have series of similar characteristics: temperature sensitivity, dose sensitivity, larva-pupal lethality and similar morphological abnormalities. Probably, there is a functional linked cluster of genes within the region 2B. Complementation groups br, rbp, l(1)pp-1, l(1)pp-2 (overlapping complex) were located by rearrangements in very narrow cytological limit 2B3-4-2B5 that is in the area of developing puff. Two other loci dor and swi are situated some to the right of 2B5.