Gut microbiome alterations in patients with wheat-dependent exercise-induced anaphylaxis
Gut microbiome alterations in patients with wheat-dependent exercise-induced anaphylaxis
复制标题
小麦依赖性运动引起的过敏反应患者肠道微生物组的改变
DOI：
10.1016/j.intimp.2020.106557
复制
发表时间：
2020-07-01
影响因子：
5.6
通讯作者：
Yin, Jia
中科院分区：
文献类型：
--
作者：
Du, Zhirong;Gao, Xiang;Yin, Jia
肠道微生物区系在食物过敏的发生发展中起着关键作用。然而，对小麦依赖型运动性过敏(WDEIA)患者肠道微生物区系的结构和组成知之甚少。我们检查了WDEIA患者的肠道微生物区系变化以及微生物区系与WDEIA的关系。收集了25例WDEIA患者和25例健康对照的粪便样本。获得环境暴露因素，测定血清总IgE、小麦、面筋蛋白和omega-5醇溶蛋白的特异性IgE。粪便样本用16S rRNA基因测序进行分析。乳杆菌属(P<；0.001)和乳杆菌属(P<；0.05)的相对丰度显著降低。WDEIA患者和健康对照组之间的微生物多样性没有差异。Omega-5醇溶蛋白特异性IgE与Oscillospira呈正相关(r=0.48，P<；0.05)，与Leuconostoc呈负相关(r=-0.49，P<；0.05)。血清总IgE水平与双歧杆菌呈显著负相关(P<；0.05)。WDEIA患者的肠道微生物组组成与健康对照组不同。我们确定了肠道微生物组和WDEIA发育之间的潜在联系。我们的发现可能会为预防和治疗WDEIA提供新的方法。
The intestinal microbiota plays a critical role in food allergy development. However, little is known regarding the structure and composition of the intestinal microbiota in patients with wheat-dependent exercise-induced anaphylaxis (WDEIA). We examined the gut microbiota alterations in patients with WDEIA and the microbiota's association with WDEIA. Fecal samples were collected from 25 patients with WDEIA and 25 healthy controls. Environmental exposure factors were obtained, serum total IgE, IgE specific to wheat, gluten, and omega-5 gliadin were measured. Fecal samples were profiled using 16S rRNA gene sequencing. The relative abundances of the bacterial genera Blautia (P < 0.05), Erysipelatoclostridium (P < 0.01), Akkermansia (P < 0.05) and Lachnospiraceae_NK4A136_group (P < 0.05) were significantly increased, while those of Lactobacillus (P = 0.001) and Dialister (P < 0.05) were significantly decreased in subjects with WDEIA. The microbial diversity did not differ between WDEIA patients and healthy controls. IgE specific to omega-5 gliadin was positively associated with the Oscillospira (r = 0.48, P < 0.05) and negatively associated with Leuconostoc (r = - 0.49, P < 0.05). Total IgE levels were significantly negatively correlated with Bifidobacterium (P < 0.05). The gut microbiome compositions in WDEIA patients differed from those of healthy controls. We identified a potential association between the gut microbiome and WDEIA development. Our findings may suggest new methods for preventing and treating WDEIA.