Identification and responsiveness of prolactin‐containing secretory granules in clonal rat mammotrophs
Identification and responsiveness of prolactin‐containing secretory granules in clonal rat mammotrophs
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克隆大鼠促乳腺激素中含催乳素分泌颗粒的鉴定和反应
DOI：
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
J. McGill
中科院分区：
文献类型：
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作者：
J. McGill
本研究使用了石川等人（1977）建立的Wister‐Imamichi大鼠胚胎Rathke囊上皮细胞的克隆垂体细胞（2B8）。发现晚期传代2B8细胞含有小分泌颗粒，其含有免疫反应性催乳素，如电子显微镜免疫细胞化学所示。当在存在已知刺激或抑制催乳素分泌的各种试剂的情况下孵育2B8细胞时，细胞质中分泌颗粒的体积密度发生改变。
Clonal pituitary cells (2B8) derived from embryonic Rathke's pouch epithelium of Wister‐Imamichi rats established by Ishikawa et al. (1977) were used in this study. Late‐passage 2B8 cells were found to contain small secretory granules which contained immunoreactive prolactin as demonstrated by electron microscopic immunocytochemistry. When 2B8 cells were incubated in the presence of various agents that are known to stimulate or inhibit prolactin secretion, the volume density of the secretory granules in the cytoplasm was shown to be altered.
胰岛素和17β-雌二醇增加GH4C1细胞的细胞内催乳素含量。
影响因子：
4.8
作者：
Kiino,DR;Dannies,PS
通讯作者：
Dannies,PS