Induction of gametogenesis in the basal cnidarian Nematostella vectensis (Anthozoa)
Induction of gametogenesis in the basal cnidarian Nematostella vectensis (Anthozoa)
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DOI：
10.1007/s00427-002-0214-7
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发表时间：
2002-03-01
影响因子：
2.4
通讯作者：
Technau, U
中科院分区：
文献类型：
--
作者：
Fritzenwanker, JH;Technau, U
建立了一个协议，可重复地诱导产卵的基础刺胞动物Nematostella vectensis（珊瑚虫）。我们发现，结合喂养制度，黑暗-光周期和温度变化协同诱导配子发生在成年息肉。雌性可产100-600个卵。这个过程可重复地导致在一年多的时间里以每周为周期生产数千个鸡蛋。配子在约2小时的时间窗口内释放，导致可预测和相当同步的发育。我们还提出了一种体外受精的方法，允许操作早期胚胎。这些方法以及简单的培养条件可以提供重要的先决条件，使用Nematostella作为模式系统的发展基础后生动物。
A protocol was established to reproducibly induce spawning in the basal cnidarian Nematostella vectensis (Anthozoa). We found that a combination of feeding regime, dark-light cycle and temperature shift synergistically induced gametogenesis in adult polyps. Females lay between 100-600 eggs. This procedure led reproducibly to the production of thousands of eggs over the course of more than I year in weekly cycles. Gametes are released in a time window of about 2 h resulting in predictable and fairly synchronized development. We also present a method for in vitro fertilization allowing manipulation of early embryos. These methods as well as the simple culture conditions could provide important prerequisites for the use of Nematostella as a model system for the development of a basal Metazoa.