PURIFICATION OF THE CENTROMERE-SPECIFIC PROTEIN CENP-A AND DEMONSTRATION THAT IT IS A DISTINCTIVE HISTONE
PURIFICATION OF THE CENTROMERE-SPECIFIC PROTEIN CENP-A AND DEMONSTRATION THAT IT IS A DISTINCTIVE HISTONE
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DOI：
10.1073/pnas.88.9.3734
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发表时间：
1991-05-01
影响因子：
11.1
通讯作者：
MARGOLIS, RL
中科院分区：
文献类型：
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作者：
PALMER, DK;ODAY, K;MARGOLIS, RL
CENP-A是一种着丝粒特异性的17-kDa蛋白，具有组蛋白样性质。 然而，与常见的体细胞组蛋白相比，CENP-A定量保留在公牛精子中，我们利用这一事实将CENP-A纯化至表观均一性。 纯化蛋白的部分序列分析表明CENP-A是一个独特的基因产物。 一些CENP-A序列与组蛋白H3的区域高度相似。 CENP-A的其他片段与H3或任何其他组蛋白无关。 这些不相关的片段可能参与CENP-A定位到着丝粒DNA或CENP-A的着丝粒特异性功能。
CENP-A, a centromere-specific 17-kDa protein, has histone-like properties. However, in contrast to the common somatic histones, CENP-A is quantitatively retained in bull spermatozoa, and we have exploited this fact to purify CENP-A to apparent homogeneity. Partial sequence analysis of the purified protein indicates that CENP-A is a distinctive gene product. Some CENP-A sequences are highly similar to regions of histone H3. Other segments of CENP-A are not related to H3 or any other histone. These unrelated segments are presumably involved in localizing CENP-A to centromeric DNA or in centromere-specific functions of CENP-A.