Selected isomiR expression profiles via arm switching?
Selected isomiR expression profiles via arm switching?
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DOI：
10.1016/j.gene.2013.09.102
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发表时间：
2014-01-01
期刊：
影响因子：
3.5
通讯作者：
Chen, Feng
中科院分区：
文献类型：
--
作者：
Guo, Li;Zhang, Hui;Chen, Feng
成熟的微小RNA（miRNA）可能由发夹状前体的5p或3p臂产生。这种选择可能通过“臂转换”而具有灵活性。然而，越来越多的证据表明，许多前体miRNA的两条臂都能产生成熟的有功能的miRNA。在此，我们试图通过分析内部的以及已发表的小RNA深度测序数据集来比较两条臂之间的异构体（isomiR）表达谱。尽管许多miR - # - 5p和miR - # - 3p已被报道为有功能的miRNA，但同时被鉴定为丰富的miRNA种类的miRNA对较少（在肿瘤细胞和正常细胞中分别收集到11对和6对）。根据异构体类型和主要异构体种类，miR - # - 5p和miR - # - 3p显示出不同的异构体表达谱以及不同的富集水平。非优势臂的异构体谱在5'端不像优势臂的异构体谱那样保守。如果miR - # - 5p和miR - # - 3p都大量表达，它们的异构体表达谱在不同样本中总是稳定的。与miR - # - 5p和miR - # - 3p的不同富集水平类似，异构体表达模式也可能受到“臂转换”现象的影响。不同的异构体表达谱进一步增加了多种异构体的复杂性，并使编码 - 非编码RNA调控网络变得复杂。（C）2013爱思唯尔公司。保留所有权利。
A mature miRNA may be generated from 5p or 3p arm of a hairpin precursor. The selection may be flexible via "arm switching". However, accumulating evidences suggest that both arms of many pre-miRNAs can yield mature functional miRNAs. Herein, we attempted to compare the isomiR expression profiles between the two arms through analyzing in-house and published small RNA deep sequencing datasets. Although many miR-#-5p and miR-#-3p have been reported as functional miRNAs, fewer miRNA pairs (11 and 6 pairs are collected in tumor and normal cells, respectively) are simultaneously identified as abundant miRNA species. According to isomiR types and dominant isomiR species, miR-#-5p and miR-#-3p show various isomiR expression profiles as well as diverse enrichment levels. IsomiR profiles of non-dominant arm are not well-conserved in 5' ends as well as isomiR profiles of dominant arm. If both the miR-#-5p and miR-#-3p are abundantly expressed, their isomiR expression profiles are always stable across different samples. Similar to diverse enrichment levels of miR-#-5p and miR-#-3p, the isomiR expression patterns may also be influenced by the phenomenon of "arm switching". The diverged isomiR expression profiles further enrich the complexity of multiple isomiRs, and complicate the coding-non-coding RNA regulatory network. (C) 2013 Elsevier B.V. All rights reserved.