The kinetics of G-CSF mobilization of CD34+ cells in healthy people
The kinetics of G-CSF mobilization of CD34+ cells in healthy people
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DOI：
10.1046/j.1365-3148.1997.d01-75.x
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发表时间：
1997-03-01
影响因子：
1.5
通讯作者：
McCullough, J
中科院分区：
文献类型：
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作者：
Stroncek, DF;Clay, ME;McCullough, J
当健康人给予粒细胞集落刺激因子（G-CSF）10天时，外周血中CD34+细胞的数量在第四天开始增加，在第六天达到最大值，然后减少。在本研究中，我们进一步定义了 CD34+ 细胞动员峰值的时间和变异性。 22名健康人每天早上皮下注射G-CSF（7.5或10μg kg（-1）天（-1）），持续5天，并在第四次（第4天）和第五次（第5天）G-CSF注射之前以及第五次注射后24小时（第6天）分析外周血CD34+细胞计数。白细胞 (WBC) 和中性粒细胞计数在第 6 天达到最高 [WBC = 43.8 +/- 13.9 x 10(9) L(-1)（平均值 +/- 1 SD），中性粒细胞 = 36.6 +/- 12.8 x 10(9) L(-1)]。相比之下，第 6 天的 CD34+ 细胞计数 (107 +/- 104 x 10(6) L(-1)) 低于第 5 天 (128 +/- 136 x 10(6) L(-1)) (P = 0.048)，但仍高于第 4 天 (60.7 +/- 40.2 x 10(6) L(-1)) (P < 0.0001）。第五次注射后 2、4 和 6 小时测量 10 名供者的 CD34+ 细胞计数，以确定计数在第 5 天和第 6 天之间是否进一步增加。第五次注射后第 5 天 2 小时血液中 CD34+ 细胞的数量 (193 +/- 277 x 10(6) L(-1)) 大于注射前的数量 (158 +/- 190 x 10(6)) L(-1))、注射后4小时(139 +/- 158 x 10(6) L(-1))和注射后6小时(170 +/- 236 x 10(6) L(-1))，但差异不显着(P = 0.29、0.25和0.45)。在第四次 G-CSF 剂量前后测量了 12 人血液中 CD34+ 细胞的数量。第 4 天注射之前，CD34+ 计数为 61 +/- 40 x 10(6) L(-1)。 2、4和6小时时的计数分别为60 +/- 40、61 +/- 29和64 +/- 30 x 10(6) L(-1)，差异不显着（0.99、P = 0.98和P = 0.73）。总之，当健康志愿者每天注射G-CSF时，动员的CD34+细胞数量在第5天最多，第6天稍少，第4天最少。如果只需要一种PBSC成分，则可以在G-CSF注射4天后在第5天收集PBSC。如果在第 5 天和第 6 天收集 PBSC 成分，则可以在收集第一个 PBSC 成分之前或之后给予第五剂。
When healthy people are given granulocyte colony stimulating factor (G-CSF) for 10 days the number of CD34+ cells in the peripheral blood begins to increase on the fourth day, reaches a maximum on the sixth day and then decreases. In this study, we further define the time and variability of peak mobilization of CD34+ cells. Twenty-two healthy people were given G-CSF (7.5 or 10 mu g kg(-1) day(-1)) subcutaneously each morning for 5 days and peripheral blood CD34+ cell counts were analysed immediately prior to the fourth (day 4) and fifth (day 5) G-CSF injection and 24 h after the fifth injection (Day 6). White blood cell (WBC) and neutrophil counts were greatest on day 6 [WBC = 43.8 +/- 13.9 x 10(9) L(-1) (mean +/- 1 SD) and neutrophils = 36.6 +/- 12.8 x 10(9) L(-1)]. In contrast the CD34+ cell counts on day 6 (107 +/- 104 x 10(6) L(-1)) were less than on day 5 (128 +/- 136 x 10(6) L(-1)) (P = 0.048) but still greater than on day 4 (60.7 +/- 40.2 x 10(6) L(-1)) (P < 0.0001). The CD34+ cell counts of 10 donors were measured 2, 4 and 6 h after the fifth injection to determine if the counts increased further between days 5 and 6. The number of CD34+ cells in the blood on day 5 2 h after the fifth injection (193 +/- 277 x 10(6) L(-1)) was greater than the number prior to the injection (158 +/- 190 x 10(6) L(-1)), 4 h post-injection (139 +/- 158 x 10(6) L(-1)) and 6 h post-injection (170 +/- 236 x 10(6) L(-1)), but the differences were not significant (P = 0.29, 0.25 and 0.45). The number of CD34+ cells in the blood of 12 people were measured before and after the fourth G-CSF dose. Prior to the day 4 injection the CD34+ count was 61 +/- 40 x 10(6) L(-1). At 2, 4 and 6 h the counts were 60 +/- 40, 61 +/- 29 and 64 +/- 30 x 10(6) L(-1) respectively, and the differences were not significant = 0.99, P = 0.98, and P = 0.73). In conclusion, when healthy volunteers are given daily G-CSF injections, the number of mobilized CD34+ cells was the greatest on day 5, slightly less on day 6 and the least on day 4. If only one PBSC component is needed, PBSCs can be collected on day 5 after only 4 days of G-CSF. If PBSC components are collected on both days 5 and 6, the fifth dose can be given either before or after the collection of the first PBSC component.