Use of reverse micellar systems for the extraction and purification of bromelain from pineapple wastes
Use of reverse micellar systems for the extraction and purification of bromelain from pineapple wastes
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DOI：
10.1016/j.biortech.2007.09.038
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发表时间：
2008-07-01
影响因子：
11.4
通讯作者：
Raghavarao, K. S. M. S.
中科院分区：
文献类型：
--
作者：
Hebbar, H. Umesh;Sumana, B.;Raghavarao, K. S. M. S.
CTAB/异辛烷/己醇/丁醇和AOT/异辛烷的反胶束系统用于从菠萝废物（核、果皮、冠和延伸茎）的粗水提取物中提取和初步纯化菠萝蛋白酶。详细研究了菠萝核心提取物的正向和反向提取工艺参数对提取效率、活性回收率和纯化倍数的影响。使用阳离子表面活性剂 CTAB 的反胶束系统，优化的岩心萃取条件使正向和反向萃取效率分别为 45% 和 62%。在这些条件下获得了相当好的菠萝蛋白酶活性回收率（106%）和纯化率（5.2倍）。使用 CTAB 系统从果皮、延伸茎和冠中进行反胶束提取，分别产生 2.1、3.5 和 1.7 的纯化倍数。在所采用的操作条件下，使用异辛烷中的阴离子表面活性剂 AOT 从延长的茎中提取并没有产生良好的结果。 (c) 2007 Elsevier Ltd. 保留所有权利。
Reverse micellar systems of CTAB/isooctane/hexanol/butanol and AOT/isooctane are used for the extraction and primary purification of bromelain from crude aqueous extract of pineapple wastes (core, peel, crown and extended stem). The effect of forward as well as back extraction process parameters on the extraction efficiency, activity recovery and purification fold is studied in detail for the pineapple core extract. The optimized conditions for the extraction from core resulted in forward and back extraction efficiencies of 45% and 62%, respectively, using reverse micellar system of cationic surfactant CTAB. A fairly good activity recovery (106%) and purification (5.2-fold) of bromelain is obtained under these conditions. Reverse micellar extraction from peel, extended stem and crown using CTAB system resulted in purification folds of 2.1, 3.5, and 1.7, respectively. Extraction from extended stem using anionic surfactant AOT in isooctane did not yield good results under the operating conditions employed. (c) 2007 Elsevier Ltd. All rights reserved.