Visualizing three-dimensional root networks using computed tomography

Visualizing three-dimensional root networks using computed tomography
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DOI:
10.1016/j.geoderma.2006.04.009
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发表时间:
2006-12-01
期刊:
影响因子:
6.1
通讯作者:
Graf, Frank
Graf, Frank
中科院分区:
农林科学1区
文献类型:
--
作者:
Kaestner, Anders;Schneebeli, Martin;Graf, Frank

文献摘要

被引文献

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我们描述了一种从计算机断层扫描生成的三维图像重建根网络的方法。根系和气土材料界面的X射线吸收非常相似,对比度极低。该方法由三个步骤组成:使用非线性扩散滤波器的对比度增强、基于Rosin方法的阈值处理以及使用形态学连通性算法提取主要特征。通过这种方法,我们还能够以高保真度重建细根,这一点通过使用噪声增加的人工图像进行的性能实验得到了证明。该方法适用于在天然冰碛土中生长 4 个月的两个桤木根的 X 射线显微断层扫描数据集。为了简化土壤材料,将裸露的根转移到装有石英砂的有机玻璃容器中。将重建的图像与真实根部的照片进行比较。 (c) 2006 Elsevier B.V. 保留所有权利。
We describe a method to reconstruct the root network from a three-dimensional image generated with computed tomography. The X-ray absorption of roots and the interface of the air-soil material is very similar and the contrast extremely low. The method consists of three steps: contrast enhancement using a non-linear diffusion filter, thresholding based on Rosin's method and extraction of the main features using a morphological connectivity algorithm. With this approach, we are also capable of reconstructing fine roots with high fidelity, which is shown with a performance experiment using artificial images with increasing noise. The method is applied to an X-ray microtomography data set of two alder roots, grown for 4months in a natural moraine soil. To simplify the soil material, the bare roots were transferred into a Plexiglas receptacle filled with quartz sand. The reconstructed image is compared with a photograph of the real roots. (c) 2006 Elsevier B.V. All rights reserved.