ROCK mediates the inflammatory response in thrombin induced microglia
ROCK mediates the inflammatory response in thrombin induced microglia
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ROCK介导凝血酶诱导的小胶质细胞的炎症反应
DOI：
10.1016/j.neulet.2013.08.065
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发表时间：
2013-10-25
影响因子：
2.5
通讯作者：
Ye, Xinchun
中科院分区：
文献类型：
--
作者：
Cui, Guiyun;Zuo, Tao;Ye, Xinchun
为了研究 ROCK 通路是否参与凝血酶诱导的小胶质细胞炎症反应，用凝血酶抑制剂阿加曲班或 ROCK 抑制剂 Y-27632 预处理凝血酶诱导的小胶质细胞。通过荧光素标记乳胶珠的吞噬作用分析和一氧化氮（NO）和肿瘤坏死因子-α（INF-α）等炎症介质的表达来评估小胶质细胞炎症反应。与非诱导的小胶质细胞相比，凝血酶诱导的小胶质细胞显示出吞噬能力显着增强，ROCK、NO 和 TNF-α 表达增加。用阿加曲班或 Y-27632 预处理凝血酶诱导的小胶质细胞显着降低吞噬能力并减少 ROCK、NO 和 INF-α 表达。因此，ROCK通路可能在凝血酶诱导小胶质细胞炎症反应的机制中发挥重要作用。 (C) 2013 Elsevier Ireland Ltd. 保留所有权利。
To investigate whether the ROCK pathway is involved in thrombin-induced microglial inflammatory response, thrombin-induced microglia were pretreated with the thrombin inhibitor argatroban or a ROCK inhibitor Y-27632. Microglial inflammatory response was evaluated by phagocytosis of fluorescein labeled latex beads analyses and inflammatory mediators' expression such as nitric oxide (NO) and tumor necrosis factor-alpha (INF-alpha). Compared to non-induced microglia, thrombin-induced microglia show significantly enhanced phagocytotic capacity and increased ROCK, NO and TNF-alpha expression. Pretreatment of thrombin-induced microglia with argatroban or Y-27632 significantly decreased phagocytotic capacity and reduced ROCK, NO and INF-alpha expression. Therefore, the ROCK pathway may play a vital role in the mechanisms by which thrombin induces microglia in the inflammatory response. (C) 2013 Elsevier Ireland Ltd. All rights reserved.