Drosophila vitelline membrane cross-linking requires the fs(1)Nasrat, fs(1)polehole and chorion genes activities
Drosophila vitelline membrane cross-linking requires the fs(1)Nasrat, fs(1)polehole and chorion genes activities
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DOI：
10.1007/s00427-001-0192-1
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发表时间：
2001-12-01
影响因子：
2.4
通讯作者：
Gargiulo, G
中科院分区：
文献类型：
--
作者：
Cernilogar, FM;Fabbri, F;Gargiulo, G
在果蝇卵黄膜形成的最后一步，构成这一层的结构蛋白通过共价键交联。在本报告中，我们分析了卵黄膜交联的突变体有缺陷，无论是在这一层或在绒毛膜层。在fs（1）Nasrat和fs（1）polehole突变等位基因中，卵黄膜形成缺陷，观察到卵黄膜交联的破坏，表明这两个基因参与了这一过程。相反，在fs（I）Nasrat和fs（l）polehole等位基因中，仅在胚胎末端显示缺陷，卵黄膜正常形成，证实了其基因产物的多功能活性。在绒毛膜蛋白基因Cp36的突变体和绒毛膜扩增突变体fs（1）K1214中也检测到卵黄膜交联的改变，表明绒毛膜层的结构成分在卵黄膜硬化过程中的作用。
During the final step of Drosophila vitelline membrane formation, the structural proteins composing this layer become cross-linked by covalent bonds. In the present report, we analyzed the vitelline membrane cross-linking in mutants having defects either in this layer or in the chorionic layers. In the fs(1)Nasrat and fs(1)polehole mutant alleles conferring defects in vitelline membrane formation, disruption of vitelline membrane cross-linking was observed, indicating the involvement of these two genes in the process. On the contrary, in the fs(I)Nasrat and fs(l)polehole alleles showing defects only at the termini of the embryo the vitelline membrane is properly formed, confirming a multifunctional activity of their gene products. Altered vitelline membrane cross-linking was also detected in a mutant of the chorion protein gene Cp36 and in the chorion amplification mutant fs(1)K1214, suggesting a role of the structural components of chorion layers in the process of vitelline membrane hardening.