Cellular uptake of liposomes targeted to intercellular adhesion molecule-1 (ICAM-1) on bronchial epithelial cells
Cellular uptake of liposomes targeted to intercellular adhesion molecule-1 (ICAM-1) on bronchial epithelial cells
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DOI：
10.1016/s0005-2736(99)00074-7
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发表时间：
1999-07-15
影响因子：
3.4
通讯作者：
Henricks, PAJ
中科院分区：
文献类型：
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作者：
Mastrobattista, E;Storm, G;Henricks, PAJ
以前，已经证明带有抗细胞间粘附分子-1（ICAM-1）抗体（mAb F10.2）的免疫脂质体可以特异性结合表达ICAM-1的不同细胞类型。在这项研究中，我们已经定量的免疫脂质体结合IFN-γ激活的人支气管上皮细胞（BEAS-2B）在体外的量，并研究了细胞结合的抗ICAM-1免疫脂质体的后续命运。我们证明，免疫脂质体与上皮细胞的结合取决于所使用的脂质体浓度。在与细胞表面结合后，抗ICAM-1免疫脂质体被上皮细胞迅速内化。60%的细胞结合免疫脂质体在37 ℃孵育1小时内被上皮细胞内化。结果表明，ICAM-1靶向免疫脂质体可用作载体，用于将抗炎药物细胞内递送至以ICAM-1表达增加为特征的炎症部位。(C)1999 Elsevier Science B. V.保留所有权利。
Previously, it was demonstrated that immunoliposomes, bearing anti-intercellular adhesion molecule-1 (ICAM-1) antibodies (mAb F10.2), can specifically bind to different cell types expressing ICAM-1. In this study, we have quantified the amount of immunoliposomes binding to IFN-gamma activated human bronchial epithelial cells (BEAS-2B) in vitro and studied the subsequent fate of cell-bound anti-ICAM-1 immunoliposomes. We demonstrate that binding of the immunoliposomes to the epithelial cells depends on the liposome concentration used. After binding to the cell surface, the anti-ICAM-1 immunoliposomes are rapidly internalised by the epithelial cells. Sixty percent of cell-bound immunoliposomes were internalised by the epithelial cells within 1 h of incubation at 37 degrees C. The results indicate that ICAM-1 targeted immunoliposomes may be used as carriers for the intracellular delivery of anti-inflammatory drugs to sites of inflammation characterised by an increased expression of ICAM-1. (C) 1999 Elsevier Science B.V. All rights reserved.