Solid-state NMR spectroscopy provides atomic-level insights into the dehydration of cartilage.
Solid-state NMR spectroscopy provides atomic-level insights into the dehydration of cartilage.
复制标题
固态核磁共振波谱提供了软骨脱水的原子级见解。
DOI：
10.1021/jp205663z
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Ramamoorthy,Ayyalusamy
中科院分区：
文献类型：
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作者：
Xu,Jiadi;Zhu,Peizhi;Morris,MichaelD;Ramamoorthy,Ayyalusamy
原子水平的洞察关节软骨的功能将有助于制定预防策略和治疗关节疾病，如骨关节炎。然而，软骨的组成和结构及其与软骨独特力学性能的关系非常复杂，对大多数生物物理技术提出了巨大的挑战。在这项研究中，我们提出了一个研究的结构和动力学的关节软骨聚合物分子在脱水过程中使用时间分辨固态核磁共振波谱。采用全层软骨外植体进行魔角旋转实验，监测刚性碳和活动碳的结构变化。我们的研究结果表明，脱水降低了糖胺聚糖中胶原氨基酸残基和碳糖环结构的流动性，但对反式xaa - pro构象没有影响。同样有趣的是，我们的研究结果表明，脱水效应是可逆的，分子结构和流动性在再水化后恢复。
An atomic-level insight into the functioning of articular cartilage would be useful to develop prevention strategies and therapies for joint diseases such as osteoarthritis. However, the composition and structure of cartilage and their relationship to its unique mechanical properties are quite complex and pose tremendous challenges to most biophysical techniques. In this study, we present an investigation of the structure and dynamics of polymeric molecules of articular cartilage using time-resolved solid-state NMR spectroscopy during dehydration. Full-thickness cartilage explants were used in magic-angle spinning experiments to monitor the structural changes of rigid and mobile carbons. Our results reveal that the dehydration reduced the mobility of collagen amino acid residues and carbon sugar ring structures in glycosaminoglycans but had no effect on thetrans-Xaa-Pro conformation. Equally interestingly, our results demonstrate that the dehydration effects are reversible, and the molecular structure and mobility are restored upon rehydration.