Expression of Small Leucine-Rich Proteoglycans in the Developing Retina and Kainic Acid-Induced Retinopathy in ICR Mice
Expression of Small Leucine-Rich Proteoglycans in the Developing Retina and Kainic Acid-Induced Retinopathy in ICR Mice
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DOI：
10.1292/jvms.10-0464
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发表时间：
2011-04-01
影响因子：
1.2
通讯作者：
Uehara, Masato
中科院分区：
文献类型：
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作者：
Ali, Safwat Ali Mohamed;Hosaka, Yoshinao Z.;Uehara, Masato
本研究的目的是确定小亮氨酸丰富的蛋白聚糖（PG），核心蛋白聚糖，双糖链蛋白聚糖和纤维调节蛋白，在ICR小鼠视网膜的发育变化，并阐明其在成人视网膜中的作用，使用红藻氨酸（KA）诱导的视网膜变性模型。取出生前、出生后和成年小鼠视网膜进行组织学和免疫组化染色，观察这些PG分布的变化。核心蛋白聚糖和纤维调节蛋白免疫染色弥漫分布在产前和产后早期阶段，在成人视网膜更强。然而，双糖链蛋白聚糖在出生前和出生后早期呈中等分布，在成年视网膜中呈微弱分布。在玻璃体内注射KA后第1、3和7天收集视网膜。注射KA的眼的视网膜发生皱缩，并伴有严重的内层损伤。与正常眼相比，KA注射眼的视网膜内层核心蛋白聚糖和纤调蛋白表达上调。我们的研究结果表明，核心蛋白聚糖和纤维调节蛋白在视网膜分化中起关键作用，并有助于视网膜损伤和修复过程。然而，双糖链蛋白聚糖在小鼠视网膜发育或修复过程中可能没有作用或仅具有有限的作用。
The aim of this study was to determine the developmental changes of small leucine-rich proteoglycans (PGs), decorin, biglycan and fibromodulin, in ICR mouse retinas and to elucidate their role in the adult retina using kainic acid (KA)-induced retinal degeneration model. Retinas, of prenatal, postnatal and adult mice were collected for histological and immunohistochemical staining to investigate the changes in distribution of these PGs. Decorin-and fibromodulin-immunostainings were diffusely distributed at prenatal and early postnatal stages and, were stronger in the adult retina. However, biglycan was moderately distributed in the prenatal and early postnatal stages and was faint in the adult retina. Retinas were collected at I, 3 and 7 days after intravitreal injection of KA. Retinas of KA injected eyes underwent shrinkage accompanied by serious damage in the inner layers. Decorin and fibromodulin were upregulated in the inner retinal layers of KA-injected eyes compared to the normal ones. Our results suggest that decorin and fibromodulin play key roles in retinal differentiation, and contribute to the retinal damage and repair process. However, biglycan may have no or only a limited role in the mouse retinal development or repair process.