THE CHALCONE SYNTHASE MULTIGENE FAMILY OF PETUNIA-HYBRIDA (V30) - DIFFERENTIAL, LIGHT-REGULATED EXPRESSION DURING FLOWER DEVELOPMENT AND UV-LIGHT INDUCTION
THE CHALCONE SYNTHASE MULTIGENE FAMILY OF PETUNIA-HYBRIDA (V30) - DIFFERENTIAL, LIGHT-REGULATED EXPRESSION DURING FLOWER DEVELOPMENT AND UV-LIGHT INDUCTION
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DOI：
10.1007/bf00020506
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发表时间：
1989-02-01
影响因子：
5.1
通讯作者：
MOL, JNM
中科院分区：
文献类型：
--
作者：
KOES, RE;SPELT, CE;MOL, JNM
我们分析了编码来自矮牵牛的类黄酮生物合成酶查尔酮合酶 (CHS) 的基因家族的 8-10 个成员的表达。在正常植物发育过程中，仅表达基因家族的两个成员（CHS-A 和 CHS-J）。它们的表达主要限于花组织。总 CHS mRNA 池中约 90% 是从 CHS-A 转录的，而 CHS-J 在花冠、管和花药中转录约 10%。 CHS-A 和 CHS-J 在花发育过程中的表达是协调的并且依赖于（红）光。在幼苗和细胞悬浮培养物中，CHS-A 和 CHS-J 的表达可以用紫外线诱导。除了 CHS-A 和 CHS-J 之外，紫外线还诱导幼苗中另外两个 CHS 基因（CHS-B 和 CHS-G）的表达，尽管水平较低。与豆科的 CHS 基因相反，矮牵牛 CHS 基因不能被植物病原体衍生的激发子诱导。在调节性 CHS 突变体矮牵牛红星中，CHS-A 和 CHS-J 的表达降低到相似的程度，表明这两个基因受到相同的反式作用因子的调节。 CHS-A 和 CHS-J 的启动子序列的比较揭示了一些惊人的同源性，这可能代表顺式作用调节序列。
We have analysed the expression of the 8-10 members of the gene family encoding the flavonoid biosynthetic enzyme chalcone synthase (CHS) from Petunia hybrida. During normal plant development only two members of the gene family (CHS-A and CHS-J) are expressed. Their expression is restricted to floral tissues mainly. About 90% of the total CHS mRNA pool is transcribed from CHS-A, whereas CHS-J delivers about 10% in flower corolla, tube and anthers. Expression of CHS-A and CHS-J during flower development is coordinated and (red) light-dependent. In young seedlings and cell suspension cultures expression of CHS-A and CHS-J can be induced with UV light. In addition to CHS-A and CHS-J, expression of another two CHS genes (CHS-B and CHS-G) is induced in young seedlings by UV light, albeit at a low level. In contrast to CHS genes from Leguminoseae, Petunia CHS genes are not inducible by phytopathogen-derived elicitors. Expression of CHS-A and CHS-J is reduced to a similar extent in a regulatory CHS mutant, Petunia hybrida Red Star, suggesting that both genes are regulated by the same trans-acting factors. Comparison of the promoter sequences of CHS-A and CHS-J reveals some striking homologies, which might represent cis-acting regulatory sequences.