Leptin enhances lung maturity in the fetal rat
Leptin enhances lung maturity in the fetal rat
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DOI：
10.1203/01.pdr.0000227478.29271.52
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发表时间：
2006-08-01
影响因子：
3.6
通讯作者：
Funanage, Vicky L.
中科院分区：
文献类型：
--
作者：
Kirwin, Susan M.;Bhandari, Vineet;Funanage, Vicky L.
肺泡11型细胞合成并分泌磷脂和表面活性蛋白。在大多数哺乳动物物种中，肺表面活性物质的磷脂和蛋白质的合成随着胎肺成熟而增加，胎肺成熟发生在妊娠后期。可能促进肺成熟和表面活性物质产生的因素包括胎盘激素，瘦素，其表达随着胎龄的增加而增加。我们证明，生理浓度的瘦素（1和10 ng/mL）的水平增加表面活性蛋白（SP）A，B，和C mRNA以及SP-A和SP-B蛋白在d-17胎鼠肺外植体在体外。为了确定瘦素是否在体内发挥类似的作用，我们产前给予瘦素怀孕的大鼠，并比较其效果的地塞米松，一个已知的调解胎儿肺发育。妊娠期给予瘦素2 d可显著增加胎肺相对于体重的平均重量。组织学分析显示，胎肺重量的增加伴随着11型肺泡细胞的数量和成熟的增加以及这些细胞中表面活性蛋白B和C的表达。总的来说，这些结果表明，瘦素是一种细胞因子调节大鼠胎肺成熟。
Pulmonary alveolar type 11 cells synthesize and secrete phospholipids and surfactant proteins. In most mammalian species, the synthesis of phospholipids and proteins of lung surfactant increases with fetal lung maturation, which occurs late in gestation. Factors that may promote lung maturation and surfactant production include the placental hormone, leptin, whose expression increases with advancing gestational age. We demonstrate that physiologic concentrations of leptin (I and 10 ng/mL) increase the levels of surfactant proteins (SP) A, B, and C mRNA as well as SP-A and SP-B protein in d-17 fetal rat lung explants in vitro. To determine whether leptin exerts similar effects in vivo, we administered leptin antenatally to pregnant rats and compared its effects to that of dexamethasone, a known mediator of fetal lung development. Antenatal treatment with leptin for 2 d significantly increased the average weight of the fetal lungs in relation to their body weight. Histologic analysis revealed that the increase in fetal lung weight was accompanied by an increase in the number and maturation of type 11 alveolar cells and the expression of surfactant proteins B and C in these cells. Collectively, these results suggest that leptin is a cytokine regulator of rat fetal lung maturity.