Functional mapping of the rat olfactory bulb using diverse odorants reveals modular responses to functional groups and hydrocarbon structural features
Functional mapping of the rat olfactory bulb using diverse odorants reveals modular responses to functional groups and hydrocarbon structural features
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DOI：
10.1002/cne.10284
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发表时间：
2002-07-22
影响因子：
2.5
通讯作者：
Leon, M
中科院分区：
文献类型：
--
作者：
Johnson, BA;Ho, SL;Leon, M
为了了解大鼠的嗅觉代码，我们收集了超过1，500，000个肾小球活动的测量值，这些测量值响应于54种气味剂，以提供官能团和烃结构的差异。每一种气味都通过不同的刺激肾小球簇（称为模块）引起了独特的反应模式。共享其结构的特定方面的气味剂激活了相同的模块，使我们能够将反应与大约80%的肾小球层的结构联系起来。最明显的关系是存在特定的含氧官能团和活动的肾小球内背模块。观察到具有广泛的烃结构，包括脂肪族，环状和芳香族特征的气味剂的官能团特异性的反应。即使是甲酸和丙酮，最简单的气味具有酸或酮官能团，分别刺激模块特定于这些官能团。同时，模式相似性的定量分析揭示了相似的烃结构的气味剂之间的活化模式的关系。气味反应是足够可靠的，使我们能够准确地预测特定方面的气味分子结构的诱发肾小球活动模式，以及预测的位置，肾小球活动诱发的新气味。
In an effort to understand the olfactory code of rats, we collected more than 1,500,000 measurements of glomerular activity in response to 54 odorants selected to provide differences in functional groups and hydrocarbon structure. Each odorant evoked a unique response pattern by differentially stimulating clusters of glomeruli, called modules. Odorants sharing specific aspects of their structure activated the same modules, allowing us to relate responses to structure across approximately 80% of the glomerular layer. The most obvious relationship was between the presence of particular oxygen-containing functional groups and the activity of glomeruli within dorsal modules. Functional group-specific responses were observed for odorants possessing a wide range of hydrocarbon structure, including aliphatic, cyclic, and aromatic features. Even formic acid and acetone, the simplest odorants possessing acid or ketone functional groups, respectively, stimulated modules specific for these functional groups. At the same time, quantitative analysis of pattern similarities revealed relationships in activation patterns between odorants of similar hydrocarbon structure. The odorant responses were reliable enough to allow us to predict accurately specific aspects of odorant molecular structure from the evoked glomerular activity pattern, as well as predicting the location of glomerular activity evoked by novel odorants.