Fetal rat lung maturation: initiation and modulation.
Fetal rat lung maturation: initiation and modulation.
复制标题
胎鼠肺成熟：启动和调节。
DOI：
10.1152/jappl.1983.55.6.1725
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Wilson,CM
中科院分区：
文献类型：
--
作者：
Gross,I;Wilson,CM
这项研究的目的是确定循环激素水平的升高是否对启动胎儿肺成熟负责。将13-20天胎鼠肺的外植体培养在含有0或1%血清的恒定化学成分的培养液中。生长、形态成熟和肺发育的两个生化标志物，饱和磷脂酰胆碱(DSPC)合成和特异性核糖皮质激素结合，表明在培养中继续发育的证据。正常情况下，在妊娠20天后，dsPC含量和胆碱掺入dspc的速率显著增加。无论所用胎儿肺的胎龄如何，在相同胎龄20天的培养中也有类似的增加(例如，6天后14天的肺，4天后16天的肺)。在培养初期切除50%或75%的肺间充质组织并不能阻止胆碱掺入的增加。由于培养环境是恒定的，而且几乎不含激素，我们得出结论，启动胎肺成熟的刺激很可能位于肺组织本身。循环荷尔蒙的作用可能是对成熟过程的后期调节。
The purpose of this study was to determine whether a rise in the level of circulating hormones is responsible for initiating fetal lung maturation. Explants of 13- to 20-day fetal rat lung were cultured in a constant chemically defined medium containing 0 or 1% serum. Growth, morphological maturation, and two biochemical markers of lung development, disaturated phosphatidylcholine (DSPC) synthesis and specific nuclear glucocorticoid binding, showed evidence of continuing development in culture. There is normally a marked increase in DSPC content and in the rate of choline incorporation into DSPC after 20 days gestation. Regardless of the gestational age of the fetal lungs used, there was a similar increase in culture at an equivalent gestational age of 20 days (e.g., 14-day lung after 6 days, 16-day lung after 4 days). Removal of 50 or 75% of the lung mesenchymal tissue at the initiation of the culture period did not prevent the increase in choline incorporation. Since the culture environment was constant and contained virtually no hormones, we conclude that the stimulus for the initiation of fetal lung maturation is most likely located in the lung tissue itself. The role of circulating hormones is probably later modulation of the maturational process.