Narrow-band UVB induces more carcinogenic skin tumors than broad-band UVB through the formation of cyclobutane pyrimidine dimer
Narrow-band UVB induces more carcinogenic skin tumors than broad-band UVB through the formation of cyclobutane pyrimidine dimer
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DOI：
10.1038/sj.jid.5701001
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发表时间：
2007-12-01
影响因子：
6.5
通讯作者：
Nishigori, Chikako
中科院分区：
文献类型：
--
作者：
Kunisada, Makoto;Kumimoto, Hiroshi;Nishigori, Chikako
窄带UVB（NB-UVB）的峰值仅在311 nm波长处，已被发现在治疗各种皮肤疾病方面比传统的宽带UVB（BB-UVB）更有效。为了评估NB-UVB和BB-UVB之间致癌活性的差异，我们通过用这两种UV源照射无毛的白化病、Ogg 1基因敲除小鼠和C57 BL/6 J野生小鼠来研究皮肤肿瘤的形成。我们发现NB-UVB诱发皮肤恶性肿瘤的比率明显高于BB-UVB。NB-UVB诱发Ogg 1基因敲除小鼠和野生型小鼠皮肤肿瘤的致癌性无显著差异。为了研究不同紫外线源引起不同致癌活性的可能原因，我们检测了三种类型的DNA损伤：环丁烷嘧啶二聚体（CPD），（6-4）光产物和8-氧代鸟嘌呤（8-oxoG）。我们发现，NB-UVB照射最小红斑剂量（MED）后CPD的形成显着高于BB-UVB照射1 MED后的CPD形成，而BB-UVB照射后（6-4）光产物和8-oxoG的形成显着高于NB-UVB照射后的CPD形成。这些结果表明CPD的形成与NB-UVB的高致癌特性密切相关。JID期刊俱乐部文章：有关本文的问题，答案和公开讨论，请访问htt：network.nature.com/。
Phototherapy with narrow-band UVB (NB-UVB), with a peak exclusively at 311nm wavelength, has been found to be more effective in treating a variety of skin diseases than conventional broad-band UVB (BB-UVB). To assess the difference in carcinogenic activity between NB-UVB and BB-UVB, we investigated skin tumor formation by irradiating albino hairless, Ogg1 knockout mice and C57BL/6J wild counterparts with these two UV sources. We found that the ratio of malignant skin tumors induced by NB-UVB was significantly higher than that induced by BB-UVB. There was no significant difference in carcinogenicity of skin tumor induced by NB-UVB between Ogg1 knockout and wild-type mice. To investigate the possible cause of different carcinogenic activity by the different UV sources, we examined three types of DNA damage: cyclobutane pyrimidine dimer (CPD), (6-4) photoproduct, and 8-oxoguanine (8-oxoG) induced by each UV source. We found that CPD formation following a minimum erythema dose (MED) by NB-UVB was significantly higher than that following 1MED by BB-UVB, whereas the formation of (6-4) photoproducts and 8-oxoG following BB-UVB was significantly higher than those following NB-UVB exposure. These results suggest that CPD formation is closely related to the higher carcinogenic characteristics of NB-UVB. JID JOURNAL CLUB ARTICLE: For questions, answers and open discussion about this article please go to htt://network.nature.com/.