Ratiometric mechanosensitive fluorescent dyes: Design and applications.
Ratiometric mechanosensitive fluorescent dyes: Design and applications.
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DOI：
10.1039/c5tc03504j
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发表时间：
2016-04-14
期刊：
影响因子：
--
通讯作者：
Theodorakis EA
中科院分区：
文献类型：
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作者：
Haidekker MA;Theodorakis EA
荧光分子对外界影响的几乎即时响应和相对低成本的测量仪器，几个世纪以来一直是有吸引力的分析工具和生物传感器。最近，先进的化学合成和靶向设计加速了荧光探针的发展。本文重点介绍了具有链段迁移率（称为荧光分子转子）的染料，它们作为机械传感器，以其发射量子产率与微粘度的关系而闻名。荧光寿命与量子产率直接相关，但稳态发射强度与量子产率无关。为了消除稳态仪器的混杂因素，可以使用双波段发射染料，并且已经开发了分子转子，其具有固有的双发射或具有非敏感的参考单元以提供校准发射波段。本文介绍了几类双发射分子转子的理论、化学结构、应用和有针对性的设计。
Fluorescent molecules, with their almost instantaneous response to external influences and relatively low-cost measurement instrumentation, have been attractive analytical tools and biosensors for centuries. More recently, advanced chemical synthesis and targeted design have accelerated the development of fluorescent probes. This article focuses on dyes with segmental mobility (known as fluorescent molecular rotors) that act as mechanosensors, which are known for their relationship of emission quantum yield with microviscosity. Fluorescence lifetime is directly related to quantum yield, but steady-state emission intensity is not. To remove confounding factors with steady-state instrumentation, dual-band emission dyes can be used, and molecular rotors have been developed that either have intrinsic dual emission or that have a non-sensitive reference unit to provide a calibration emission band. We report on theory, chemical structure, applications and targeted design of several classes of dual-emission molecular rotors.