DETECTION OF LASER-UV MICRO-IRRADIATION-INDUCED DNA PHOTOLESIONS BY IMMUNOFLUORESCENT STAINING
DETECTION OF LASER-UV MICRO-IRRADIATION-INDUCED DNA PHOTOLESIONS BY IMMUNOFLUORESCENT STAINING
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DOI：
10.1007/bf00279058
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发表时间：
1980-01-01
期刊：
影响因子：
5.3
通讯作者：
NAKANISHI, K
中科院分区：
文献类型：
--
作者：
CREMER, C;CREMER, T;NAKANISHI, K
使用波长257 nm的低功率激光-紫外微束对中国仓鼠细胞核的一小部分进行显微照射。在间期或随后的中期固定后，用远紫外光处理的 DNA 光产物的抗血清进行间接免疫荧光染色。紫外线照射 DNA 特异性抗体可用于直接检测激光紫外线微照射引起的 DNA 光损伤。讨论了该方法对于研究间期核中染色体空间排列的潜在用途。
A low-power laser-UV microbeam of wavelength 257 nm was used for microirradiation of a small part of the nucleus of Chinese hamster cells. Following fixation in interphase or in the subsequent metaphase, indirect immunofluorescent staining was performed with antiserum to photoproducts of DNA treated with far UV light. Antibodies specific for UV-irradiated DNA can be used for a direct detection of laser-UV microirradiation-induced DNA photolesions. The potential usefulness of this method for investigation of the spatial arrangement of chromosomes in the interphase nucleus is discussed.