Glycophase glass revisited: protein adsorption and cell growth on glass surfaces bearing immobilized glycerol monosaccharides.
Glycophase glass revisited: protein adsorption and cell growth on glass surfaces bearing immobilized glycerol monosaccharides.
复制标题
重新审视糖相玻璃：带有固定化甘油单糖的玻璃表面上的蛋白质吸附和细胞生长。
DOI：
10.1016/s0142-9612(02)00035-2
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发表时间：
2002
期刊：
影响因子：
14
通讯作者：
Hoffman,AllanS
中科院分区：
文献类型：
--
作者：
Bain,JamesR;Hoffman,AllanS
γ-甘油基丙基甲硅烷基或“glycophase”玻璃已被推广为抗蛋白质吸附和细胞附着的无污染表面，在其上可以吸附寡肽配体，从而产生细胞类型特异性培养表面。本研究证实，富含甘油的糖相表面是一个有用的支持肽固定。但观察到glycophase glass比glass吸附更多的白蛋白。在pH 7.4下，解吸研究表明，白蛋白与血型玻璃的结合比与玻璃的结合更紧密。此外，转基因βG I/17胰岛素瘤细胞培养物在两种表面上的生长速率、形态和功能是等同的。Glycophase glass既不排斥蛋白质，也不排斥细胞。
γ-Glycerylpropylsilyl or “glycophase” glass has been promoted as a non-fouling surface, resistant to protein adsorption and cell attachment, on which one can immobilize oligopeptide ligands, and thus create cell-type-specific culture surfaces. The present study confirmed that the glycerol-rich glycophase surface is a useful support for peptide immobilization. But glycophase glass was observed to adsorb more albumin than glass. At pH 7.4, desorption studies revealed that albumin bound more tightly to glycophase glass than to glass. Moreover, the growth rates, morphologies, and functions of transgenic βG I/17 insulinoma cell cultures were equivalent on the two surfaces. Glycophase glass is neither protein- nor cell-repellant.