Community ecology of hot spring cyanobacterial mats: predominant populations and their functional potential
Community ecology of hot spring cyanobacterial mats: predominant populations and their functional potential
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DOI：
10.1038/ismej.2011.73
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发表时间：
2011-08-01
期刊：
影响因子：
11
通讯作者：
Ward, David M.
中科院分区：
文献类型：
--
作者：
Klatt, Christian G.;Wood, Jason M.;Ward, David M.
通过鸟枪法宏基因组测序研究了蘑菇泉和章鱼泉（Yellowstone国家公园，WY，USA）流出通道中来自60 ℃和65 ℃区域的光养微生物垫群落。组装宏基因组序列的分析解决了六个占主导地位的chlorophototrophic人口，并允许发现和表征未描述的，但占主导地位的社区成员和他们的生理潜力。系统发育标记基因和功能基因的连锁表明，新的chlorophotototrophic细菌属于未知的谱系内的目的Chlorobiales和王国Chloroflexi。后者是第一个Chloroflexi王国的成员，位于单系组Chloroflexales的目的chlorophotototrophs。未组装的宏基因组序列的代表性菌株的基因组的直接比较显示了广泛的遗传多样性，基因组重排和新的生理潜力，在本地人口相比，基因组参考。聚球藻属宏基因组序列与聚球藻属的参考基因组显示出高度的共线性。菌株A和B '的同源性随序列相关性的降低而降低。有证据表明，本地人口之间的水平基因转移，但这些事件的频率成反比的系统发育相关性。The ISME Journal（2011）5，1262-1278; doi：10.1038/ismej.2011.73; 2011年6月23日在线发表
Phototrophic microbial mat communities from 60 degrees C and 65 degrees C regions in the effluent channels of Mushroom and Octopus Springs (Yellowstone National Park, WY, USA) were investigated by shotgun metagenomic sequencing. Analyses of assembled metagenomic sequences resolved six dominant chlorophototrophic populations and permitted the discovery and characterization of undescribed but predominant community members and their physiological potential. Linkage of phylogenetic marker genes and functional genes showed novel chlorophototrophic bacteria belonging to uncharacterized lineages within the order Chlorobiales and within the Kingdom Chloroflexi. The latter is the first chlorophototrophic member of Kingdom Chloroflexi that lies outside the monophyletic group of chlorophototrophs of the Order Chloroflexales. Direct comparison of unassembled metagenomic sequences to genomes of representative isolates showed extensive genetic diversity, genomic rearrangements and novel physiological potential in native populations as compared with genomic references. Synechococcus spp. metagenomic sequences showed a high degree of synteny with the reference genomes of Synechococcus spp. strains A and B', but synteny declined with decreasing sequence relatedness to these references. There was evidence of horizontal gene transfer among native populations, but the frequency of these events was inversely proportional to phylogenetic relatedness. The ISME Journal (2011) 5, 1262-1278; doi: 10.1038/ismej.2011.73; published online 23 June 2011