Analysis of Plasma Hydrogen Sulfide, Homocysteine, and L-Cysteine in Open-Angle Glaucoma Patients
Analysis of Plasma Hydrogen Sulfide, Homocysteine, and L-Cysteine in Open-Angle Glaucoma Patients
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DOI：
10.1089/jop.2020.0023
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发表时间：
2020-06-03
影响因子：
2.3
通讯作者：
Zhong, Yisheng
中科院分区：
文献类型：
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作者：
Lin, Zhongjing;Huang, Shouyue;Zhong, Yisheng
目的：比较原发性开角型青光眼(POAG)、正常眼压性青光眼(NTG)、高眼压性青光眼(OHT)和正常人血浆硫化氢(H 2S)、同型半胱氨酸(Hcy)和L半胱氨酸(Cys)水平。目的：探讨青光眼的相关因素，评价其对青光眼的诊断能力。方法：选择2016-12/2018-12瑞金医院收治的POAG、NTG、OHT和正常人。所有受试者均进行了全面的眼科检查，并抽取空腹静脉血测定血浆中硫化氢、同型半胱氨酸和环磷酰胺的浓度。结果：入选POAG组42例，NTG组20例，OHT组52例，对照组78例。POAG组的H_2S水平显著低于OHT组(P=0.036)和正常组(P<；0.001)，而POAG和NTG组的同型半胱氨酸和半胱氨酸水平显著高于正常对照组(P=0.007~0.043)。不同分期青光眼患者血清中H_2S、Hcy、Cys浓度差异无统计学意义(均P>；0.05)。诊断病程越长的POAG患者血药浓度越低(P=0.026，P=0.001，P<；0.001)，而NTG患者血药浓度差异无统计学意义(P>0.05)。正常对照组同型半胱氨酸、半胱氨酸水平与平均偏差呈负相关(r=-0.450，P=0.047；r=-0.478，P=0.033)。所有这些浓度在POAG正常组中均有显著的诊断价值[曲线下面积=0.642-0.721，P<；0.05]。在POAG-OHT组中，H_2S水平对青光眼的诊断能力较差(AUC=0.657，P<；0.01)。结论：H_2S水平降低、Hcy和Cys水平升高可能与青光眼有关，尤其是在POAG中。然而，H2S/Hcy代谢途径不够敏感，不能作为青光眼的可靠生物标志物。
Purpose: To compare the plasma levels of hydrogen sulfide (H2S), homocysteine (Hcy), and L-cysteine (Cys) among primary open-angle glaucoma (POAG), normal tension glaucoma (NTG), ocular hypertension (OHT), and normal individuals. To explore associated factors and evaluate their diagnostic abilities in glaucoma.Methods: POAG, NTG, OHT, and normal subjects were recruited from Ruijin Hospital between December 2016 and December 2018. All subjects underwent thorough ophthalmological examinations, and fasting venous blood was taken to determine the concentrations of H2S, Hcy, and Cys.Results: Forty-two POAG, 20 NTG, 52 OHT, and 78 controls were enrolled. The H2S levels in POAG group were significantly lower than those in OHT group (P = 0.036) and normal group (P < 0.001), while the Hcy and Cys levels in POAG and NTG groups were significantly higher (P = 0.007-0.043). The concentrations of H2S, Hcy, and Cys in glaucoma patients with different stages were not significantly different (all P > 0.05). POAG patients with longer duration of diagnosis had lower concentrations (P = 0.026, P = 0.001, P < 0.001), but no significant differences in NTG patients (all P > 0.05). The Hcy and Cys levels in NTG patients showed negative but weak correlations with mean deviation (r = -0.450, P = 0.047; r = -0.478, P = 0.033). All these concentrations showed significant but poor diagnostic values in POAG-Normal group [area under curve (AUC) = 0.642-0.721, P < 0.05]. The H2S level showed poor diagnostic power in POAG-OHT group (AUC = 0.657, P < 0.01).Conclusion: Decreased levels of H2S and increased levels of Hcy and Cys may be associated with glaucoma, especially in POAG. However, the H2S/Hcy metabolic pathway is not sufficiently sensitive to be used as a reliable biomarker in glaucoma.