Transforming growth factor-β1, insulin-like growth factors, and insulin-like growth factor binding proteins in ovarian follicular fluid are differentially regulated by the type of ovarian hyperstimulation used for in vitro fertilization
Transforming growth factor-β1, insulin-like growth factors, and insulin-like growth factor binding proteins in ovarian follicular fluid are differentially regulated by the type of ovarian hyperstimulation used for in vitro fertilization
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DOI：
10.1016/s0015-0282(98)00105-8
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发表时间：
1998-07-01
影响因子：
6.7
通讯作者：
Tally, M
中科院分区：
文献类型：
--
作者：
Fried, G;Wramsby, H;Tally, M
目的：确定 hMG 和高纯度 FSH 对卵泡生成转化生长因子-β(1)（TGF-β(1)）、胰岛素样生长因子 I 和 II（IGF-I 和 IGF-II）以及胰岛素样生长因子结合蛋白-1 和 -3（IGFBP-1 和 IGFBP-3）的影响。设计：对照临床研究。背景：大学 IVF 计划。患者：120 名女性目前不孕持续 3 年的患者参与了这项研究。干预措施：在取卵时收集卵泡液和匹配血清，并使用 ELISA 和 RIA 分析生长因子和 E-2。主要结果指标：卵泡液中 TGF-β(1)、IGF-I、IGF-II、IGFBP-1 和 IGFBP-3 水平以及血清中 E-2 水平结果：与高度纯化的 FSH 相比，hMG 过度刺激卵巢产生的 TGF-β(1) 和 IGF-I 水平较低，而 IGFBP-1 水平较高。两种治疗的 IGF-II 和 IGFBP-3 水平相似。 结论：在接受 IVF 的患者中，与含有 1:1 比例的 FSH 和 LH 的制剂 (hMG) 相比，高纯度 FSH 对滤泡中 TGF-β(1)、IGF-I 和 IGFBP-1 的表达进行了不同的调节。结果表明，FSH 和 LH 不同地控制卵巢产生这些生长因子。 （Fertil Steril(R) 1998；70:129-34 (C) 1998，美国生殖医学会。）。
Objective: To determine the effects of hMG and highly purified FSH on follicular production of the ovarian growth factors transforming growth factor-beta(1) (TGF-beta(1)), insulin-like growth factors I and II (IGF-I and IGF-II), and insulin-like growth factor binding proteins-1 and -3 (IGFBP-1 and IGFBP-3).Design: Controlled clinical study.Setting: University IVF program.Patient(s): One hundred twenty women who were 3-year duration of infertility in their present relationship participated in the study.Intervention(s): Follicular fluid and matched serum were collected at oocyte pick-up and analyzed for growth factors and E-2 with the use of ELISA and RIA.Main Outcome Measure(s): Levels of TGF-beta(1), IGF-I, IGF-II, IGFBP-1, and IGFBP-3 in follicular fluid and levels of E-2 in serum were measured.Result(s): Compared with highly purified FSH, ovarian hyperstimulation with hMG produced lower levels of TGF-beta(1) and IGF-I and higher levels of IGFBP-1. Levels of IGF-II and IGFBP-3 were similar with the 2 treatments.Conclusion(s): In patients undergoing IVF, the follicular expression of TGF-beta(1), IGF-I, and IGFBP-1 was regulated differently by highly purified FSH compared with a preparation containing FSH and LH in a 1:1 ratio (hMG). The results indicate that FSH and LH control ovarian production of these growth factors differentially. (Fertil Steril(R) 1998;70:129-34 (C) 1998 by American Society for Reproductive Medicine.).