Subunit composition, protein interactions, and structures of the mammalian brain sec6/8 complex and septin filaments
Subunit composition, protein interactions, and structures of the mammalian brain sec6/8 complex and septin filaments
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DOI：
10.1016/s0896-6273(00)80493-6
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发表时间：
1998-06-01
期刊：
影响因子：
16.2
通讯作者：
Scheller, RH
中科院分区：
文献类型：
--
作者：
Hsu, SC;Hazuka, CD;Scheller, RH
在酵母中，sec6/8复合体和隔膜细丝都参与了将囊泡和蛋白质引导到活性膜加成部位的过程。大鼠脑Sec6/8复合体免疫共沉淀物与由四个哺乳动物Septin组成的细丝共沉淀，提示这些复合体之间存在相互作用。其中一种分隔素CDC10显示出广泛的亚细胞和组织分布，在有丝分裂后神经元和分裂细胞中都有发现。电子显微镜研究表明，纯化的大鼠脑间隔蛋白形成直径为8.25 nm的细丝，长度为25 nm的倍数。戊二醛固定的大鼠脑Sec6/8复合体采用类似字母“T”或“Y”的构象。Sec6/8和Septin复合体可能在运输小泡和组织神经元质膜上的蛋白质方面发挥重要作用。
Both the sec6/8 complex and septin filaments have been implicated in directing vesicles and proteins to sites of active membrane addition in yeast. The rat brain sec6/8 complex coimmunoprecipitates with a filament composed of four mammalian septins, suggesting an interaction between these complexes. One of the septins, CDC10, displays broad subcellular and tissue distributions and is found in postmitotic neurons as well as dividing cells. Electron microscopic studies showed that the purified rat brain septins form filaments of 8.25 nm in diameter; the lengths of the filaments are multiples of 25 nm. Glutaraldehyde-fixed rat brain sec6/8 complex adopts a conformation resembling the letter "T" or "Y". The sec6/8 and septin complexes likely play an important role in trafficking vesicles and organizing proteins at the plasma membrane of neurons.