DISTINCT REACTIVITIES OF 4 MONOCLONAL-ANTIBODIES WITH HUMAN INTERLEUKIN-2 RECEPTOR
DISTINCT REACTIVITIES OF 4 MONOCLONAL-ANTIBODIES WITH HUMAN INTERLEUKIN-2 RECEPTOR
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DOI：
10.1111/j.1348-0421.1985.tb02960.x
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发表时间：
1985-01-01
影响因子：
2.6
通讯作者：
HINUMA, Y
中科院分区：
文献类型：
--
作者：
TANAKA, Y;TOZAWA, H;HINUMA, Y
两种新的鼠单克隆IgG 1抗体，H-31和H-A26，与先前获得的两种抗人白细胞介素2（IL-2）受体（IL-2 R）的单克隆抗体，anti-Tac和HIEI进行了比较。在用各种人造血细胞进行的免疫荧光测定中，H-31和H-A26抗体均仅与IL-2 R阳性细胞反应，它们沉淀IL-2 R分子，即分子量为60 K和53 K道尔顿的糖蛋白（gp 60/gp 53），来自携带人T细胞白血病病毒I型（HTLV-I）的MT-2细胞，如用抗Tac抗体吸收IL-2 R后的连续免疫沉淀所证实的。抗体结合竞争试验表明，H-31和抗-Tac，和H-A26和HIEI，分别，竞争结合细胞，抗-Tac也抑制HIEI的结合，但反之亦然。H-31与抗Tac一样，强烈抑制正常活化T细胞的IL-2依赖性增殖、IL-2的吸收和IL-2与细胞的直接结合，而H-A26与HIEI一样，仅微弱抑制这些过程。这些抗体与HTLV-I感染的各种猿猴细胞系的反应谱是不同的，如免疫荧光研究所示。显示人IL-2 R表达用HIEI检测到的独特抗原决定簇，其在旧世界猴和新世界猴的IL-2 R分子中不可检测，并且还表达猿IL-2 R分子共有的决定簇。这些观察结果表明，H-31和H-A26识别人IL-2 R分子，并且由H-31、H-A26、抗Tac和HIEI定义的IL-2 R分子上的抗原位点是不同的。
Two new murine monoclonal IgGI antibodies, H-31 and H-A26, were characterized in comparison with two previously obtained monoclonal antibodies against human interleukin 2 (IL-2) receptor (IL-2 R), anti-Tac and HIEI. In immunofluorescence assays with various human hematopoietic cells, H-31 and H-A26 antibodies both reacted with only IL-2 R-positive cells, and they precipitated IL-2 R molecules, glycoproteins with molecular weights of 60K and 53K daltons (gp60/gp53), from human T-cell leukemia virus type I (HTLV-I)-carrying MT-2 cells, as demonstrated by sequential immunoprecipitation after absorption of IL-2 R with anti-Tac. Antibody-binding competition assays showed that H-31 and anti-Tac, and H-A26 and HIEI, respectively, competed reciprocally in binding to the cells, and that anti-Tac also inhibited the binding of HIEI but not vice versa. H-31, like anti-Tac, strongly inhibited the IL-2-dependent proliferation of normal activated T-cells, absorption of IL-2 and direct binding of IL-2 to the cells, while H-A26, like HIEI, inhibited those processes only weakly. The spectra of reactivities of these antibodies with various simian cell lines derived by HTLV-I infection were different, as revealed by immunofluorescence studies. Human IL-2 R was shown to express a unique antigenic determinant, detected with HIEI, that was not detectable in IL-2 R molecules of Old and New World monkeys, and also to express determinants common to simian IL-2 R molecules. These observations indicate that H-31 and H-A26 recognize human IL-2 R molecules and that the antigenic sites on the IL-2 R molecule defined by H-31, H-A26, anti-Tac, and HIEI are different.