Colored moderately thermophilic bacteria in paper-machine biofilms
Colored moderately thermophilic bacteria in paper-machine biofilms
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DOI：
10.1007/s10295-003-0047-z
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发表时间：
2003-04-01
影响因子：
3.4
通讯作者：
Salkinoja-Salonen, MS
中科院分区：
文献类型：
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作者：
Kolari, M;Nuutinen, J;Salkinoja-Salonen, MS
生物膜在造纸中引起几个问题。本文报道了对六台造纸机和纸板机中彩色生物膜的微生物调查，包括两个发现致病菌的彩色粘液爆发的案例研究，共分离出95株粉红色、红色、橙子或黄色的菌株。几乎所有（99%）的菌株在52 ℃生长，72%在56 ℃生长，但只有30%在28 ℃生长，表明大多数菌株是中度嗜热的。用微量滴定板测定法分析了菌株的生物膜形成潜力和杀菌剂敏感性。在5 ppm的亚甲基双硫氰酸酯或2，2-二溴-3-次氮基丙酰胺存在下，在造纸机水中，55株形成生物膜。此外，在杀生物剂的存在下，39种菌株使生物膜产生增加5-753%，这表明抑制粘附细胞但不抑制表面附着细胞的杀生物剂浓度实际上可能促进生物结垢。细胞可能已经灭活了一部分杀生物剂，因为该测定中的细胞密度高，对应于循环沃茨中出现的最高细胞密度。从几个米尔斯分离到四组有色细菌。粉红色的Deinococcus geothermalis和红色的Meiothermus silvanus是造纸机中常见的主要生物膜形成菌。这份报告是第一次描述的Meiothermus物种在造纸工业中的红泥形成的参与。第三组细菌（假定与玫瑰单胞菌属有关的新物种）包含的菌株不是生物膜形成者，但通常在中性或碱性机器的粘液中发现。第四组含有红色色素的生物膜形成菌株，代表与红细菌属相关的α-变形菌门的新属。许多彩色造纸机细菌是以前从温泉的微生物垫中发现的物种。这里描述了细菌群的一些特征，以便于在未来造纸工业中发生彩色粘液爆发的情况下识别它们。
Biofilms cause several problems in papermaking. This report describes a microbiological survey of colored biofilms in six paper and board machines, including two case studies of outbreaks of colored slimes in which the causative bacteria were found. A total of 95 pink-, red-, orange- or yellow-pigmented strains were isolated. Nearly all (99%) of the strains grew at 52 degreesC, 72% grew at 56 degreesC, but only 30% grew at 28 degreesC, indicating that most of the strains were moderately thermophilic. Biofilm formation potential and biocide susceptibility of the strains were analyzed with a microtiter plate assay. In the presence of 5 ppm of methylene bisthiocyanate or 2,2-dibromo-3-nitrilopropionamide in paper-machine water, 55 strains formed biofilms. Moreover, 39 strains increased biofilm production by 5-753% in the presence of biocide, suggesting that biocide concentrations inhibitory to planktonic but not to surface-attached cells may actually promote biofouling. The cells may have inactivated a portion of the biocides, as the cell density in this assay was high, corresponding to the highest cell densities occurring in the circulating waters. Four groups of colored bacteria that were isolated from several mills were identified. Pink-pigmented Deinococcus geothermalis and red-pigmented Meiothermus silvanus occurred as common primary biofilm-formers in paper machines. This report is the first description of the involvement of Meiothermus species in red-slime formation in the paper industry. The third group of bacteria (putative new species related to Roseomonas) contained strains that were not biofilm formers, but which were commonly found in slimes of neutral or alkaline machines. The fourth group contained red-pigmented biofilm-forming strains representing a novel genus of alpha-Proteobacteria related to Rhodobacter. Many colored paper-machine bacteria are species previously known from microbial mats of hot springs. Some characteristics of the bacterial groups are described here in order to facilitate their recognition in future cases of colored-slime outbreaks in the paper industry.