Mitochondrial DNA Alterations in Colorectal Cancer Cell Lines
Mitochondrial DNA Alterations in Colorectal Cancer Cell Lines
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DOI：
10.1272/jnms.78.13
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发表时间：
2011-02-01
影响因子：
1
通讯作者：
Ohta, Shigeo
中科院分区：
文献类型：
--
作者：
Chihara, Naoto;Amo, Taku;Ohta, Shigeo
线粒体DNA（mtDNA）的体细胞突变已在不同类型的癌症中被报道，并且被认为在转移、癌症发展和对抗癌剂的反应中起作用。为了预测mtDNA改变在结直肠癌中的潜在作用，我们测定了11个人源性结直肠癌细胞系的mtDNA全序列，并与修订后的剑桥参考序列进行比较，以确定核苷酸改变。发现四种同质改变和六种异质改变是新颖的。其中，同源G6709 A（MT-CO 1）和G14804 A（MT-CYB）的改变导致高度保守残基的氨基酸变化。异质性G1576 A（MT-RNR 1）和G2975 A（MT-RNR 2）的改变预计会使线粒体核糖体RNA的茎结构不稳定。这些核苷酸改变是可能在癌症中发挥重要作用的候选者。（J Nippon Med Sch 2011：78：13-21）
Somatic mutations of mitochondrial DNA (mtDNA) have been reported in different types of cancers and are suggested to play roles in metastasis, cancer development and response to anticancer agents. To predict potential roles of mtDNA alterations in colorectal cancer, we determined the entire mtDNA sequence of eleven human-derived colorectal cancer cell lines and compared with the revised Cambridge Reference Sequence to identify nucleotide alterations. Four homoplasmic and six heteroplasmic alterations were found to be novel. Among them, homoplasmic G6709A (MT-CO1) and G14804A (MT-CYB) alterations cause amino acid changes in the highly conserved residues. Heteroplasmic G1576A (MT-RNR1) and G2975A (MT-RNR2) alterations are expected to make the stem structure of mitochondrial ribosomal RNAs unstable. These nucleotide alterations are candidates that could play important roles in cancer. (J Nippon Med Sch 2011: 78: 13-21)