BLOCKADE OF NMDA-RECEPTORS PREVENTS OCULARITY CHANGES IN KITTEN VISUAL-CORTEX AFTER REVERSED MONOCULAR DEPRIVATION
BLOCKADE OF NMDA-RECEPTORS PREVENTS OCULARITY CHANGES IN KITTEN VISUAL-CORTEX AFTER REVERSED MONOCULAR DEPRIVATION
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DOI：
10.1016/0165-3806(89)90183-1
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发表时间：
1989-06-01
期刊：
影响因子：
--
通讯作者：
SINGER, W
中科院分区：
文献类型：
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作者：
GU, Q;BEAR, MF;SINGER, W
我们在猫的纹状皮质中研究了反向遮盖后单眼剥夺的恢复是否需要激活N-甲基-D-天冬氨酸(NMDA)受体。3~4周龄小猫右眼用眼睑缝线闭合1周。随后，这只眼睛被重新睁开，左眼皮缝合了另一周。在第二周期间，将NMDA受体拮抗剂2-氨基-5-磷酸戊酸(APV)从渗透性微泵注入左侧视皮层(50nmol/h)，而右侧视皮层仅注入溶剂(生理盐水)作为对照。第二周末，用单单位记录法评价纹状皮质神经元的眼优势。在对照侧大脑半球，绝大多数神经元由新睁开的眼睛控制，而在APV治疗的半球，大多数神经元仍由新剥夺的眼睛控制。此外，APV处理的大脑半球中的神经元反应较差，方向调谐减少。这些数据证实，皮层NMDA受体的慢性阻断破坏了单眼剥夺后剥夺通路的断开，并降低了皮质神经元的反应性和定向选择性。此外，他们还表明，阻断NMDA受体也阻止了反向闭塞后剥夺通路的视觉依赖恢复。
We investigated in the striate cortex of kittens whether the recovery from the effects of monocular deprivation that occurs after reverse occlusion requires activation of N-methyl-D-aspartate (NMDA) receptors. The right eye of 3-4-week-old kittens was closed by lid suture for one week. Subsequently this eye was reopened and the left eyelid sutured closed for another week. During this second week, the NMDA-receptor antagonist, 2-amino-5-phosphonovaleric acid (APV), was infused from an osmotic minipump into the left visual cortex (50 nmol/h), while the right visual cortex was infused only with vehicle solution (saline) as control. At the end of the second week, the ocular dominance of striate cortical neurons was assessed with single unit recording. In the control hemispheres, the large majority of neurons was dominated by the newly opened eye, while in the APV-treated hemispheres most neurons were still dominated by the newly deprived eye. In addition, neurons in the APV-treated hemispheres were less responsive and showed a reduction of orientation tuning. These data confirm that chronic blockade of cortical NMDA-receptors disrupts the disconnection of deprived pathways after monocular deprivation and reduces both responsiveness and orientation selectivity of cortical neurons. In addition they indicate that blockade of NMDA-receptors prevents also vision-dependent recovery of deprived pathways after reverse occlusion.