Pixel-wise programmability enables dynamic high-SNR cameras for high-speed microscopy.
Pixel-wise programmability enables dynamic high-SNR cameras for high-speed microscopy.
复制标题
逐像素可编程性可实现用于高速显微镜的动态高信噪比相机。
DOI：
10.1101/2023.06.27.546748
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Wilson,Matthew
中科院分区：
文献类型：
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作者：
Zhang,Jie;Newman,Jonathan;Wang,Zeguan;Qian,Yong;Guo,Wei;Chen,ZheSage;Linghu,Changyang;Etienne-Cummings,Ralph;Fossum,Eric;Boyden,Edward;Wilson,Matthew
高速宽视场荧光显微镜有潜力以卓越的时空分辨率捕获生物过程。然而，传统相机在高帧速率下存在低信噪比的问题，限制了它们检测微弱荧光事件的能力。在这里，我们介绍了一种图像传感器，其中每个像素都具有单独可编程的采样速度和相位，因此可以安排像素以高信噪比同时高速采样。在高速电压成像实验中，与低噪声科学 CMOS 相机相比，我们的图像传感器显着提高了输出信噪比（约 2-3 倍）。这种信噪比增益能够检测标准科学 CMOS 相机遗漏的微弱神经元动作电位和阈下活动。我们的相机具有灵活的像素曝光配置，可提供多种采样策略，以提高各种实验条件下的信号质量。
High-speed wide-field fluorescence microscopy has the potential to capture biological processes with exceptional spatiotemporal resolution. However, conventional cameras suffer from low signal-to-noise ratio at high frame rates, limiting their ability to detect faint fluorescent events. Here, we introduce an image sensor where each pixel has individually programmable sampling speed and phase, so that pixels can be arranged to simultaneously sample at high speed with a high signal-to-noise ratio. In high-speed voltage imaging experiments, our image sensor significantly increases the output signal-to-noise ratio compared to a low-noise scientific CMOS camera (~2–3 folds). This signal-to-noise ratio gain enables the detection of weak neuronal action potentials and subthreshold activities missed by the standard scientific CMOS cameras. Our camera with flexible pixel exposure configurations offers versatile sampling strategies to improve signal quality in various experimental conditions.