Study of the impact of cranberry extract on the virulence factors and biofilm formation by Enterococcus faecalis strains isolated from urinary tract infections
Study of the impact of cranberry extract on the virulence factors and biofilm formation by Enterococcus faecalis strains isolated from urinary tract infections
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蔓越莓提取物对尿路感染粪肠球菌毒力因子和生物膜形成影响的研究
DOI：
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
A. Hendrich
中科院分区：
文献类型：
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作者：
D. Wojnicz;Dorota Tichaczek;K. Korzekwa;M. Kicia;A. Hendrich
摘要蔓越莓果汁的饮用和含蔓越莓提取物的商业制剂的应用被推荐用于预防和治疗尿路感染（UTIs），特别是对于复发性UTIs的妇女。许多研究集中在蔓越莓对尿路致病性大肠杆菌（E。coli）菌株。然而，蔓越莓对革兰氏阳性粪肠球菌（E。粪便）是有限的。因此，我们研究的目的是确定商业浓缩蔓越莓提取物对大肠杆菌生长、毒力因子和生物膜形成的活性。从尿液中分离的粪杆菌菌株。采用肉汤微量稀释法测定蔓越莓提取物的最小抑菌浓度（MIC）。药敏试验采用纸片扩散法。测定了蔓越莓提取物对细菌存活、疏水性、脂肪酶、卵磷脂酶、DNA酶、溶血素、明胶酶的合成以及生物膜质量的影响。结果表明，蔓越莓提取物抑制细菌的生长、酶活性并限制生物膜的形成。所研究的蔓越莓提取物的抗菌活性证实其可成功用于预防由E.粪便。
Abstract Drinking of cranberry fruit juice and application of commercial preparations containing the cranberry extracts are recommended in the prevention and treatment of urinary tract infections (UTIs), especially in women with recurrent UTIs. Many studies focus on the activity of cranberries against uropathogenic Escherichia coli (E. coli) strains. However, the knowledge of the cranberry effect on Gram-positive Enterococcus faecalis (E. faecalis) is limited. Therefore, the aim of our study was to establish the activity of commercial concentrated cranberry extract on the growth, virulence factors and biofilm formation of E. faecalis strains isolated from urine. Minimal inhibitory concentrations (MICs) of cranberry extract were determined by the broth microdilution method. Disc diffusion method was used to determine antimicrobial susceptibility. The impact of cranberry extract on bacterial survival, hydrophobicity, synthesis of lipase, lecithinase, DNase, hemolysin, gelatinase and biofilm mass was determined. Results show that cranberry extract inhibits the growth, enzymatic activities of bacteria and limits biofilm formation. The antibacterial activities of the studied cranberry extract confirm that it could be successfully used in prevention of UTIs caused by E. faecalis.