Castration induces apoptosis in the mouse epididymis during postnatal development
Castration induces apoptosis in the mouse epididymis during postnatal development
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DOI：
10.1507/endocrj.49.75
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发表时间：
2002-02-01
影响因子：
2
通讯作者：
Terada, N
中科院分区：
文献类型：
--
作者：
Takagi-Morishita, Y;Kuhara, A;Terada, N
本实验观察了去势对小鼠生后发育过程中附睾细胞凋亡的影响。附睾重量从出生第0天（出生当天）至出生后第20天缓慢增加，随后迅速增加。在第0、5、10、20、30、40和60天的去势增加了附睾头（头）、体（体）和尾（尾）上皮细胞的凋亡指数（凋亡细胞的百分比），它们的凋亡指数在去势后第2天达到最大水平，但在去势后第60天的尾中在第4天达到最大凋亡指数。去势后第0、5、10、20天的头部、体部和尾部的最大凋亡指数显著低于去势后第40、60天的凋亡指数。从去势后第2天的附睾中提取的DNA在琼脂糖凝胶电泳上显示梯形图，这是凋亡的特征。当丙酸睾酮（10 μ g/g体重）每天两次注射到已在第10、30或60天去势的小鼠中时，附睾的头部、身体和尾部的凋亡指数的增加被完全抑制。去势后6天，第0、5、10、20、30、40和60天的配对附睾重量显著低于假手术小鼠的重量，表明睾丸从出生到成年都在分泌雄激素。目前的研究结果表明，雄激素剥夺引起的去势诱导小鼠从出生到成年的附睾上皮细胞凋亡，并建议上皮细胞的比例，其生存依赖于睾丸，是较小的附睾在一个缓慢的生长阶段比在附睾后的这个阶段。
The effect of castration on apoptosis in the mouse epididymis during postnatal development was examined. The weight of the epididymis slowly increased from day 0 (day of birth) to day 20 after birth, followed by a rapid increase thereafter. Castration on days 0, 5, 10, 20, 30, 40 and 60 increased apoptotic indices (percentages of apoptotic cells) of epithelia of the caput (head), corpus (body), and cauda (tail) epididymis, their apoptotic indices reaching maximal levels on day 2 after castration with the exception of a maximal apoptotic index on day 4 in the tail after castration on day 60. The maximal levels of apoptotic indices of the head, body and tail after castration on days 0, 5, 10 and 20 were significantly lower than those after castration on days 40 and 60. DNAs extracted from the epididymides 2 days after castration on days 0, 5, 10 and 60 showed a ladder pattern on agarose gel electrophoresis, which is a characteristic of apoptosis. When testosterone propionate (10 mug/g body weight) was injected twice a day into mice which had been castrated on day 10, 30 or 60, the increases in apoptotic indices of the head, body and tail of the epididymis were completely inhibited. The weights of the paired epididymides 6 days after castration on days 0, 5, 10, 20, 30, 40 and 60 were significantly lower than those of sham-operated mice, indicating the secretion of androgen by the testes from birth to adulthood. The present results indicated that androgen deprivation caused by castration induces apoptosis in the epithelium of the epididymis of mice from birth to adulthood, and suggested that a proportion of epithelial cells, the survival of which is dependent on the testes, is smaller in the epididymides during a slow growth stage than in the epididymides after this stage.