Genetic variation and association mapping of aphid (Macrosiphoniella sanbourni) resistance in chrysanthemum (Chrysanthemum morifolium Ramat.)
Genetic variation and association mapping of aphid (Macrosiphoniella sanbourni) resistance in chrysanthemum (Chrysanthemum morifolium Ramat.)
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菊花 (Chrysanthemum morifolium Ramat.) 蚜虫 (Macrosiphoniella sanbourni) 抗性的遗传变异和关联图谱
DOI：
10.1007/s10681-017-2085-z
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发表时间：
2018-02-01
期刊：
影响因子：
1.9
通讯作者：
Guan, Zhiyong
中科院分区：
文献类型：
--
作者：
Fu, Xiao;Su, Jiangshuo;Guan, Zhiyong
菊花大管蚜是影响菊花观赏品质和产量的主要害虫，开发抗虫棉新品种十分重要。然而，到目前为止，菊花抗蚜虫的遗传机制还没有得到彻底的研究。本研究旨在研究温室条件下夏秋两季全球收集的80份菊花种质资源的抗蚜性遗传变异，并利用关联作图方法寻找抗蚜性的分子标记。以平均为害指数表示的抗虫性在两个季节间呈显著正相关(r=0.93，P<；0.01)。表型和遗传变异系数约为26-27%，估计了较高的广义遗传力(0.93)和适度的相对遗传进步(~68%)。以群体结构和亲缘关系矩阵为协变量的MLM模型进行关联作图，识别出11个与抗蚜相关的标记，其各自解释的表型变异在~11%~~57%之间。在两个季节预测的3个标记中，SSR184-1和E1M5-1被鉴定为抗蚜的有利等位基因。7个含有这两个有利等位基因的品种被鉴定为潜在的供体亲本，可用于未来对棉铃虫的抗性改良。这些发现进一步加深了对抗蚜性的遗传决定的理解，所确定的有利等位基因和供体亲本为未来培育抗虫性更强的菊花提供了可能性。
Aphid, Macrosiphoniella sanbourni, is a major insect pest that adversely affects ornamental quality and production of chrysanthemum, thus it is critical to develop new cultivars resistant to aphid. However, the genetic mechanism governing aphid resistance is thus far not thoroughly investigated in chrysanthemum. This study aimed to characterize the genetic variation of the aphid resistance in a global collection of 80 chrysanthemum entries, during summer and autumn under greenhouse condition, and to identify the molecular markers for aphid resistance by association mapping. The performances of aphid resistance, quantified by the average damage index of aphid, was significantly correlated (r = 0.93, P < 0.01) between two seasons. The coefficients phenotypic and genetic variation was calculated around 26–27%; and a high magnitude (0.93) of broad-sense heritability, together with a moderate relative genetic advance (~ 68%), was estimated for aphid resistance. By using the MLM model that integrates population structure and kinship matrix as covariates association mapping identified 11 markers related to aphid resistance, with the individually explained phenotypic variation ranging from ~ 11 to ~ 57%. Of the three markers predicted in both seasons, SSR184-1 and E1M5-1were identified as favorable alleles for aphid resistance. Seven cultivars harboring the two favorable alleles were identified as potential donor parents for future improvement of resistance against aphid. These findings add further understanding of the genetic determination of aphid resistance, and the identified favorable alleles and donor parents open a possibility to produce chrysanthemums with enhanced aphid resistance in future.