High-throughput microfabricated CE/ESI-MS: automated sampling from a microwell plate.
High-throughput microfabricated CE/ESI-MS: automated sampling from a microwell plate.
复制标题
DOI：
10.1021/ac001432v
复制
发表时间：
2001-04
影响因子：
7.4
通讯作者：
B. Zhang;F. Foret;B. Karger
中科院分区：
文献类型：
--
作者：
B. Zhang;F. Foret;B. Karger
提出了一种从微孔板上自动进样的高通量微细管电泳质谱(CE/ESI-MS)的新设计。该方法结合了进样口、分离通道和液结，后者用于将装置耦合到带有微型大气下电喷雾接口的MS。该微型装置连接在聚碳酸酯歧管上，带有外部电极储存池，可用于电动和压力-流体控制。每次运行后，使用计算机激活的电动气动分配器从微孔板装载样品和冲洗通道。从微器件结构中移除电极和样品储存库大大简化了芯片设计，并且不再需要钻取存取孔和样品/缓冲器储存库。外部歧管还允许使用系统延长运行时间所需的相对较大的储液器。介绍了使用该微加工系统对多肽和蛋白质消化进行自动化CE/ESI-MS分析的初步结果。
A new design for high-throughput microfabricated capillary electrophoresis/electrospray mass spectrometry (CE/ ESI-MS) with automated sampling from a microwell plate is presented. The approach combines a sample-loading port, a separation channel, and a liquid junction, the latter for coupling the device to the MS with a miniaturized subatmospheric electrospray interface. The microdevice was attached to a polycarbonate manifold with external electrode reservoirs equipped for electrokinetic and pressure-fluid control. A computer-activated electropneumatic distributor was used for both sample loading from the microwell plate and washing of channels after each run. Removal of the electrodes and sample reservoirs from the microdevice structure significantly simplified the chip design and eliminated the need both for drilling access holes and for sample/buffer reservoirs. The external manifold also allowed the use of relatively large reservoirs that are necessary for extended time operation of the system. Initial results using this microfabricated system for the automated CE/ESI-MS analysis of peptides and protein digests are presented.