The Arabidopsis thaliana dihydroxyacetone phosphate reductase gene SUPPRESSOR OF FATTY ACID DESATURASE DEFICIENCY1 is required for glycerolipid metabolism and for the activation of systemic acquired resistance
The Arabidopsis thaliana dihydroxyacetone phosphate reductase gene SUPPRESSOR OF FATTY ACID DESATURASE DEFICIENCY1 is required for glycerolipid metabolism and for the activation of systemic acquired resistance
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DOI：
10.1105/tpc.016907
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发表时间：
2004-02-01
期刊：
影响因子：
11.6
通讯作者：
Shah, J
中科院分区：
文献类型：
--
作者：
Nandi, A;Welti, R;Shah, J
系统获得性抗性（systemic acquired resistance，SAR）是植物中的一种广谱抗性机制，其在另一器官暴露于坏死性病原体后在幼稚器官中被激活。出现SAR的器官表现出水杨酸（SA）水平和病原学相关1（PR 1）基因表达的增加。SA信号是SAR表现所必需的。我们在这里证明，拟南芥抑制脂肪酸去饱和酶缺陷1（sfd 1）突变妥协的SAR赋予增强抗假单胞菌maculicola。此外，sfd 1突变减少了SAR相关的积累水平升高的SA和PR 1基因转录物在植物的远端叶先前暴露于avirullent病原体。然而，在接种病原菌的sfd 1的叶片中，对P. erichingae的强毒株和无毒毒株的基础抗性以及SA和PR 1基因转录物的升高水平的积累并没有受到损害。此外，SA功能类似物苯并噻二唑的应用增强了sfd 1突变体植物的抗病性。SFD 1编码一个假定的二羟丙酮磷酸（DHAP）还原酶，补充甘油-3-磷酸营养缺陷型的DHAP还原酶缺陷型大肠杆菌gpsA突变体。在sfd 1突变体植物中质体甘油脂质组成发生改变，表明SFD 1参与脂质代谢，并且SFD 1产物脂质对SAR的激活很重要。
Systemic acquired resistance (SAR) is a broad-spectrum resistance mechanism in plants that is activated in naive organs after exposure of another organ to a necrotizing pathogen. The organs manifesting SAR exhibit an increase in levels of salicylic acid (SA) and expression of the PATHOGENESIS-RELATED1 (PR1) gene. SA signaling is required for the manifestation of SAR. We demonstrate here that the Arabidopsis thaliana suppressor of fatty acid desaturase deficiency1 (sfd1) mutation compromises the SAR-conferred enhanced resistance to Pseudomonas syringae pv maculicola. In addition, the sfd1 mutation diminished the SAR-associated accumulation of elevated levels of SA and PR1 gene transcript in the distal leaves of plants previously exposed to an avirullent pathogen. However, the basal resistance to virulent and avirulent strains of P. syringae and the accumulation of elevated levels of SA and PR1 gene transcript in the pathogen-inoculated leaves of sfd1 were not compromised. Furthermore, the application of the SA functional analog benzothiadiazole enhanced disease resistance in the sfd1 mutant plants. SFD1 encodes a putative dihydroxyacetone phosphate (DHAP) reductase, which complemented the glycerol-3-phosphate auxotrophy of the DHAP reductase-deficient Escherichia coli gpsA mutant. Plastid glycerolipid composition was altered in the sfd1 mutant plant, suggesting that SFD1 is involved in lipid metabolism and that an SFD1 product lipid(s) is important for the activation of SAR.