MICROPATTERNING PROTEINS AND SYNTHETIC PEPTIDES ON SOLID SUPPORTS - A NOVEL APPLICATION FOR MICROELECTRONICS FABRICATION TECHNOLOGY
MICROPATTERNING PROTEINS AND SYNTHETIC PEPTIDES ON SOLID SUPPORTS - A NOVEL APPLICATION FOR MICROELECTRONICS FABRICATION TECHNOLOGY
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DOI：
10.1021/bp00014a010
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发表时间：
1992-03-01
影响因子：
2.9
通讯作者：
MOORES, G
中科院分区：
文献类型：
--
作者：
BRITLAND, S;PEREZARNAUD, E;MOORES, G
在本文中，我们描述了一种在固体载体上以微米尺寸固定蛋白质和合成多肽的方法。采用了微电子制造技术，并将其用于光刻指导蛋白质在适当衍生化表面上的固定位置。例如，我们对牛血清白蛋白（BSA）蛋白和辣根过氧化物酶（HRP）酶进行了微图像化处理。结果表明，与载体交联后，HRP的催化活性保持不变。当与固相肽合成相结合时，该技术允许合成肽再次以微米尺寸的模式构建。合成的多肽，聚赖氨酸，以尺寸接近光学光刻在1-2-mum分辨率的实际极限的模式构建。用组织化学方法结合光镜和荧光显微镜观察固定化分子和合成肽的形态。这里所描述的蛋白质和肽图谱技术是生物电子学领域的一个进步。特别是，现在应该有可能设计出与生物系统相连接的新方法，并构建用于集成到小型化生物传感器中的新设备。
In this paper, we describe a method for immobilizing proteins and synthesizing peptides in micrometer-dimension patterns on solid supports. Microelectronics fabrication technology was adapted and used to lithographically direct the location of immobilization of proteins on appropriately derivatized surfaces. As examples, we micropatterned the protein bovine serum albumin (BSA) and the enzyme horseradish peroxidase (HRP). The catalytic activity of HRP was shown to be retained after being cross-linked to the support. When coupled with solid-phase peptide synthesis, the technique allowed synthetic peptides to be constructed in patterns again having micrometer dimensions. Synthetic polypeptides, polylysine, were constructed in patterns with dimensions that approached the practical limit of resolution for optical lithography at 1-2-mum. The patterns of immobilized molecules and synthetic peptides were visualized using histochemical methods together with light and fluorescence microscopy. The protein and peptide patterning technique described here is an advance in the field of bioelectronics. In particular, it should now be possible to devise novel methods for interfacing with biological systems and constructing new devices for incorporation into miniaturized biosensors.