[Plasma bradykinin concentration in patients with cardiovascular diseases].
[Plasma bradykinin concentration in patients with cardiovascular diseases].
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心血管疾病患者血浆缓激肽浓度[J].
DOI：
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
T. Nishino
中科院分区：
文献类型：
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作者：
M. Minami;H. Togashi;M. Sano;T. Endoh;H. Saito;F. Hashimoto;K. Fujita;H. Yasuda;Y. Kuriyamoto;T. Nishino
本研究采用一种新开发的放射免疫分析技术对各种心血管疾病患者的循环激肽进行定量，并对该方法的临床应用进行评价。为了测定缓激肽(BK)的含量，本方法使用注射了Kallidin的兔抗血清。该方法具有良好的比活性、回收率和重现性。为了避免激动素的形成并阻止其失活，用装有抑制剂混合物(EDTA、Trasylol、1-10-菲咯啉、大豆胰酶抑制剂、聚薄荷烯)的聚丙烯注射器采集人体血液样本。1)正常人、高血压病患者、劳力性心绞痛患者和其他心脏病患者的血浆BK浓度分别为12.2、9.2、8.0和14.0pg/ml。(2)卡托普利后30min(12.5 mg，P.O.)给药后，血压和肺楔压降低，心输出量增加，同时血浆肾素活性、血浆BK浓度和血浆去甲肾上腺素浓度升高。3)在冷加压试验中，正常人血浆BK浓度和血压均升高，而高血压患者血浆BK浓度下降，血压升高。这种测定血浆BK的放射免疫法使测定各种心脏病患者的血浆BK浓度成为可能。
The present study was undertaken to quantify the circulating kinins in patients with various cardiovascular diseases using a newly developed radioimmunoassay technique and to evaluate this method in terms of its clinical application. For the determination of bradykinin (BK), this assay uses a rabbit anti-serum which has been injected with kallidin. This assay shows good specific activity, recovery and reproducibility. In order to avoid the formation of kinin as well as to block its inactivation, human blood samples were collected with a polypropylene syringe containing an inhibitor mixture (EDTA, trasylol, 1-10-phenanthroline, soybean trypsin inhibitor, polybrene). 1) The plasma BK concentration in normal human subjects, in patients with essential hypertension, effort angina and other cardiac diseases were 12.2, 9.2, 8.0 and 14.0 pg/ml, respectively. 2) Thirty min after captopril (12.5 mg, p.o.) administration, blood pressure and pulmonary wedge pressures decreased, and cardiac output increased accompanied with increases in plasma renin activity, plasma BK concentration and plasma norepinephrine concentration. 3) During the cold pressor test, both plasma BK concentration and blood pressure increased in the normal human subjects, whereas plasma BK levels decreased and blood pressure increased in the patients with hypertension. This radioimmunoassay for plasma BK determination makes it possible to measure plasma BK concentration in patients with various cardiac diseases.