Anatomical Coupling of Sensory and Motor Nerve Trajectory via Axon Tracking
Anatomical Coupling of Sensory and Motor Nerve Trajectory via Axon Tracking
复制标题
DOI：
10.1016/j.neuron.2011.06.021
复制
发表时间：
2011-07
期刊：
影响因子：
16.2
通讯作者：
Liang Wang;R. Klein;B. Zheng;Till Marquardt
中科院分区：
文献类型：
--
作者：
Liang Wang;R. Klein;B. Zheng;Till Marquardt
这是一个长期存在的问题，如何开发运动神经元和感觉神经元投射，共同形成一个共同的主要周围神经通路网格，传递行为输出和体感输入。在这里，我们通过小鼠的靶向细胞谱系和基因操作，结合体外活体轴突成像来探索这个问题。在缺乏运动投射的情况下，背侧（外轴）和腹侧（下轴）感觉投射以随机方式形成，而去除外轴运动轴突表达的 EphA3/4 受体酪氨酸激酶会触发选择性失败，形成外轴感觉投射。 EphA3/4 通过诱导感觉轴突沿着预先形成的外轴运动投影进行跟踪，从而非细胞自主地发挥作用。这涉及感觉轴突上的同源肝配蛋白-A 蛋白，但独立于运动轴突中的 EphA3/4 信号传导。因此，周围神经通路的组装涉及将感觉投射与离散运动通路耦合的运动轴突亚型特异性信号。
It is a long-standing question how developing motor and sensory neuron projections cooperatively form a common principal grid of peripheral nerve pathways relaying behavioral outputs and somatosensory inputs. Here, we explored this issue through targeted cell lineage and gene manipulation in mouse, combined with in vitro live axon imaging. In the absence of motor projections, dorsal (epaxial) and ventral (hypaxial) sensory projections form in a randomized manner, while removal of EphA3/4 receptor tyrosine kinases expressed by epaxial motor axons triggers selective failure to form epaxial sensory projections. EphA3/4 act non-cell-autonomously by inducing sensory axons to track along preformed epaxial motor projections. This involves cognate ephrin-A proteins on sensory axons but is independent from EphA3/4 signaling in motor axons proper. Assembly of peripheral nerve pathways thus involves motor axon subtype-specific signals that couple sensory projections to discrete motor pathways.