BIOCHEMICAL ADAPTATION OF MITOCHONDRIA, MUSCLE, AND WHOLE-ANIMAL RESPIRATION TO ENDURANCE TRAINING
BIOCHEMICAL ADAPTATION OF MITOCHONDRIA, MUSCLE, AND WHOLE-ANIMAL RESPIRATION TO ENDURANCE TRAINING
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DOI：
10.1016/0003-9861(81)90312-x
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发表时间：
1981-01-01
影响因子：
3.9
通讯作者：
BROOKS, GA
中科院分区：
文献类型：
--
作者：
DAVIES, KJA;PACKER, L;BROOKS, GA
通过运动训练的实验干预，研究线粒体生物合成，以及亚细胞、细胞和整体动物能量学的生理整合。总线粒体组成不变，大鼠肌肉耐力跑台运行10周的计划。Fe-S团簇、细胞色素、黄素蛋白、过氧化物酶、氧化酶、膜蛋白和脂质的浓度以及各组分与其他组分的比例保持恒定。肌肉的线粒体含量增加了约0.05。绝对组织氧化能力也是100%。线粒体的可溶性部分保持恒定的总蛋白质含量和质量，相对于膜部分，尽管适应性变化的一些柠檬酸循环酶的具体活动。来自耐力训练肌肉的线粒体产生正常的跨膜电位、ADP/O比率和呼吸控制比率。肌肉氧化酶活性与耐力高度相关（r = 0.92），提高了403%。整个动物的最大O2消耗（≥ VO 2 max）仅增加14%，并且是耐力的相对较差的预测因子。因此，线粒体因子，而不是VO 2 max，必须在决定耐力活动的极限中起重要作用。相反，VO 2 max与有氧运动可达到的最大工作强度强相关（r = 0.82）。在线粒体、肌肉和整个动物水平上的O2消耗的比较揭示，最大肌肉氧化酶活性不是对VO 2 max的绝对限制：其它因素可能干预以控制在运动期间可利用的肌肉O2消耗能力的百分比。
The experimental intervention of exercise training was used to study mitochondrial biosynthesis, and the physiologic integration of subcellular, cellular and whole-animal energetics. Gross mitochondrial composition was unchanged in rat muscle by a 10 wk program of endurance treadmill running. The mitochondrial concentration of Fe-S clusters, cytochromes, flavoprotein, dehydrogenases, oxidases and membrane protein and lipid, as well as the ratios of each component to the others, maintained constant proportions. The mitochondrial content of muscle increased by .apprx. 100% as did absolute tissue oxidative capacity. The soluble portions of mitochondria maintained a constant total protein content and mass, relative to the membrane fraction, despite adaptive changes in the specific activities of some citric acid-cycle enzymes. Mitochondria from endurance-trained muscles generated normal transmembrane potentials, ADP/O ratios and respiratory control ratios. Muscle oxidase activity was highly correlated (r = 0.92) with endurance capacity, which increased 403%. Whole-animal maximal O2 consumption (.ovrhdot.VO2 max), increased only 14% and was a relatively poor predictor of endurance. Thus, mitochondrial factors, rather than .ovrhdot.VO2 max, must play an important role in dictating the limits of endurance activity. Conversely, .ovrhdot.VO2 max was strongly related to the maximal intensity of work which could be attained aerobically (r = 0.82). Comparison of O2 consumption at the mitochondrial, muscle and whole-animal levels revealed that maximal muscle oxidase activity was not an absolute limitation to .ovrhdot.VO2 max: Other factors probably intervene to control the percentage of muscle O2 consumption capacity which may be utilized during exercise.