QTL analysis of fruit quality in fresh market tomato:: a few chromosome regions control the variation of sensory and instrumental traits
QTL analysis of fruit quality in fresh market tomato:: a few chromosome regions control the variation of sensory and instrumental traits
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DOI：
10.1093/jxb/erf058
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发表时间：
2002-10-01
影响因子：
6.9
通讯作者：
Buret, M
中科院分区：
文献类型：
--
作者：
Causse, M;Saliba-Colombani, V;Buret, M
番茄果实的感官质量涉及一系列属性（风味，香气，质地），可以通过感官分析或仪器测量进行评估。为了研究这一特性的遗传控制，一个重组自交系（RIL）人口开发从种内杂交樱桃番茄线具有良好的整体香气强度和自交系具有中等风味，但更大的水果。共评价了38个感官品质性状。物理性状包括果实重量、直径、颜色、硬度和弹性。化学性状包括干物质重、可滴定酸、pH值、可溶性固形物、糖、番茄红素、胡萝卜素和12种香气成分。一组经过培训的评估人员量化了感官属性：风味（甜味和酸味），香气（整体香气强度，以及糖果，柠檬，柑橘类水果和药物香气）和质地（坚固性，柔和性，粉状，多汁性和难以吞咽的皮肤）。RILs表现出很大的变化范围。利用分子标记对38个性状共定位了130个数量性状位点。它们主要分布在少数染色体区域。检测到了影响果实质量、直径、色泽、硬度、成熟度和6种香气成分的主效QTL（R-2> 30%）。利用QTL图谱分析了感官性状与仪器测量值之间的关系。对QTL与多个性状相关的少数区域提供了特殊的见解。几个例子来说明如何同步分析QTL分离相关性状可能有助于了解品质性状的遗传控制，并为QTL表征铺平道路。
The organoleptic quality of tomato fruit involves a set of attributes (flavour, aroma, texture) that can be evaluated either by sensory analyses or by instrumental measures. In order to study the genetic control of this characteristic, a recombinant inbred line (RIL) population was developed from an intraspecific cross between a cherry tomato line with a good overall aroma intensity and an inbred line with medium flavour but bigger fruits. A total of 38 traits involved in organoleptic quality were evaluated. Physical traits included fruit weight, diameter, colour, firmness, and elasticity. Chemical traits were dry matter weight, titratable acidity, pH, and the contents of soluble solids, sugars, lycopene, carotene, and 12 aroma volatiles. A panel of trained assessors quantified sensory attributes: flavour (sweetness and sourness), aroma (overall aroma intensity, together with candy, lemon, citrus fruit, and pharmaceutical aromas) and texture (firmness, meltiness, mealiness, juiciness, and skin difficult to swallow). RILs showed a large range of variation. Molecular markers were used to map a total of 130 quantitative trait loci (QTL) for the 38 traits. They were mainly distributed in a few chromosome regions. Major QTLs (R-2 >30%) were detected for fruit weight, diameter, colour, firmness, meltiness, and for six aroma volatiles. The relationships between instrumental measures and sensory traits were analysed with regard to the QTL map. A special insight was provided about the few regions where QTLs are related to multiple traits. A few examples are shown to illustrate how the simultaneous analysis of QTL segregation for related traits may aid in understanding the genetic control of quality traits and pave the way towards QTL characterization.