Differentiation and Metabolic Interrogation of Human Adipocytes
Differentiation and Metabolic Interrogation of Human Adipocytes
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DOI：
10.1007/978-1-4939-6820-6_7
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发表时间：
2017-01-01
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影响因子：
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通讯作者：
O'Rourke, Robert W.
中科院分区：
文献类型：
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作者：
Baker, Nicki A.;Muir, Lindsey A.;O'Rourke, Robert W.
由前脂肪细胞分化而来的脂肪细胞为人类脂肪细胞代谢的研究提供了一个有价值的模型。我们描述了人类间质血管细胞的分离、前脂肪细胞的扩增、向成熟脂肪细胞的分化以及脂肪细胞的体外代谢询问的方法。
Adipocytes differentiated from preadipocytes provide a valuable model for the study of human adipocyte metabolism. We describe methods for isolation of human stromal vascular cells, expansion of preadipocytes, differentiation into mature adipocytes, and in vitro metabolic interrogation of adipocytes.