Large‐scale Production of Mammalian Cells and Their Products: Engineering Principles and Barriers to Scale‐up
Large‐scale Production of Mammalian Cells and Their Products: Engineering Principles and Barriers to Scale‐up
复制标题
哺乳动物细胞及其产品的大规模生产：工程原理和扩大规模的障碍
DOI：
10.1111/j.1749-6632.1983.tb47912.x
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发表时间：
1983
影响因子：
5.2
通讯作者：
A. Sinskey
中科院分区：
文献类型：
--
作者：
M. Glacken;R. Fleischaker;A. Sinskey
哺乳动物细胞产品具有巨大的医学和临床重要性，但迄今为止，大规模生产这些产品所采用的生产方法并不具有应有的成本效益。过程控制的实施将大大提高这些产品的生产率。最近开发的大规模生产细胞的方法，如微载体、人工毛细管、管状螺旋膜和微胶囊，必须进行优化，并且必须解决氧传输限制的问题。潜在有毒废物的积累会抑制生长并降低生产力。通过调整补料分批培养的环境参数，使细胞的新陈代谢不再产生这些化合物，或者通过在培养物中持续灌注培养基，可以减少这种影响。如果这些技术障碍能够被克服，那么哺乳动物细胞衍生产品的生产成本就可以大大降低。
Mammalian cell products have great medical and clinical importance, but to date, production methods employed to manufacture these products on a large scale are not as cost efficient as they could be. The implementation of process control would greatly improve the productivity of these products. Recently developed methods to produce cells on a large scale, such as microcarriers, artificial capillaries, tubular spiral film, and microencapsulation must be optimized, and the problem of oxygen transfer limitation must be solved. The accumulation of potentially toxic waste products can inhibit growth and reduce productivity. This effect can be reduced by either adjusting the environmental parameters of a fed-batch culture, so that the cell's metabolism is shifted away from producing these compounds, or by continually perfusing medium through the culture. If these technical barriers can be overcome, the cost of producing products derived from mammalian cells can be greatly reduced.
影响因子：
20.3
作者：
Ritz,J;Schlossman,SF
通讯作者：
Schlossman,SF